P-Selectin Kit ELISA

N° du produit ABIN411351

Le kit ELISA P-Selectin (ABIN411351) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain P-Selectin. Ce kit ELISA a été cité dans 4 publications.

Aperçu rapide pour P-Selectin Kit ELISA (ABIN411351)

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Épitope: AA 42-771

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Détail du produit P-Selectin Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human P-Selectin / CD62P ELISA Kit to quantitate Human SELP in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: W42-A771

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 4.7%, 6.7%, 5.6%
Inter-Assay Precision (CV%): 6.4%, 7.8%, 5.6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour P-Selectin Kit ELISA (ABIN411351)

Fayed, Abdel-Hady, Hafez, Salama, Al-Tonbary: "Study of platelet activation, hypercoagulable state, and the association with pulmonary hypertension in children with β-thalassemia." dans: Hematology/oncology and stem cell therapy, (2017) (PubMed).

Li, Ben, Yuan, Liu, Wu, Xu, Zhang: "Recruitment of HIV-1 target cells at topical mucosal sites: a sensitive and early marker for determining the safety of microbicide candidates." dans: Emerging microbes & infections, Vol. 2, Issue 7, pp. e42, (2015) (PubMed).

Entezari-Maleki, Hajhossein Talasaz, Salarifar, Hadjibabaie, Javadi, Bozorgi, Jenab, Boroumand, Gholami: "Plasma Vitamin D Status and Its Correlation with Risk Factors of Thrombosis, P-selectin and hs-CRP Level in Patients with Venous Thromboembolism; the First Study of Iranian Population." dans: Iranian journal of pharmaceutical research : IJPR, Vol. 13, Issue 1, pp. 319-27, (2014) (PubMed).

Zhong, He, Yang, Bao, Zhang, Zhou, Chen, Li, Han, Yang, Sun, Cao, Li, Shi, Jiang, Zhang, Yan: "L-selectin and P-selectin are novel biomarkers of cervicovaginal inflammation for preclinical mucosal safety assessment of anti-HIV-1 microbicide." dans: Antimicrobial agents and chemotherapy, Vol. 56, Issue 6, pp. 3121-32, (2012) (PubMed).

Détail du antigène

Antigène: P-Selectin (SELP) (Selectin P (Granule Membrane Protein 140kDa, Antigen CD62) (SELP))

Autre désignation: SELP

Sujet:

Background: P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa.

Gene Full Name: selectin P

ID gène: 6403

UniProt: P16109