TIMP3 Kit ELISA

N° du produit ABIN411360

Le kit ELISA TIMP3 (ABIN411360) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain TIMP3. Ce kit ELISA a été cité dans 1 publication.

Aperçu rapide pour TIMP3 Kit ELISA (ABIN411360)

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Épitope: AA 24-211

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva

Détail du produit TIMP3 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Human TIMP-3 ELISA Kit to quantitate Human TIMP3 in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: C24-P211

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 2 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7%, 6.8%, 6.2%
Inter-Assay Precision (CV%): 8.5%, 8.2%, 8.1%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour TIMP3 Kit ELISA (ABIN411360)

Denkovskij, Bagdonas, Kusleviciute, Mackiewicz, Unguryte, Porvaneckas, Fleury, Venalis, Jorgensen, Bernotiene: "Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases and Their Inhibitors in the Osteoarthritic Cartilage In Vitro." dans: Stem cells international, Vol. 2017, pp. 9542702, (2017) (PubMed).

Détail du antigène

Antigène: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Autre désignation: TIMP3

Sujet:

Background: The tissue inhibitors of metalloproteinases (TIMPs) are natural inhibitors of the matrix metalloproteinases, a group of zinc-binding endopeptidases involved in the degradation of the extracellular matrix. The TIMP3 gene is expressed in many tissues, with highest expression in the placenta. TIMP3 encodes a potent angiogenesis inhibitor and is mutated in Sorsby fundus dystrophy, a macular degenerative disease with submacular choroidal neovascularization. TIMP3 gene is mapped to 22q12.1-q13.2. Mutations in TIMP3 cause the autosomal dominant disorder Sorsby's fundus dystrophy (SFD).

Gene Full Name: TIMP metallopeptidase inhibitor 3

ID gène: 7078

UniProt: P35625