VCAM1 Kit ELISA

N° du produit ABIN411368

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Souris VCAM1. Il a été cité dans 6 publications.

Aperçu rapide pour VCAM1 Kit ELISA (ABIN411368)

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Épitope: AA 25-698

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Détail du produit VCAM1 Kit ELISA

Seuil minimal de détection: 156 pg/mL

Fonction: Sandwich Mouse VCAM-1 ELISA Kit to quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: F25-E698

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 5 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.8%, 7.2%, 6.8%
Inter-Assay Precision (CV%): 8.2%, 8.7%, 7.7%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour VCAM1 Kit ELISA (ABIN411368)

Zhang, Mu, Zhou, Song, Zhang, Wu, Jiang, Wang, Zhang, Li, Shao, Wang, Li, Yang, Wu, Zhang, Tang: "Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction." dans: PLoS ONE, Vol. 11, Issue 6, pp. e0158038, (2017) (PubMed).

Xu, Li, Dong, Tan, Wu, Xie, Yang: "Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE-/- Mice through the ROS/MAPK/NF-κB Pathway." dans: Nutrients, Vol. 7, Issue 8, pp. 7085-105, (2016) (PubMed).

Barros, Borges, Ferreira, Carmo, Rosado, Fouque, Mafra: "Is there interaction between gut microbial profile and cardiovascular risk in chronic kidney disease patients?" dans: Future microbiology, Vol. 10, Issue 4, pp. 517-26, (2015) (PubMed).

Huang, Wang, Liu, Zheng, Li: "Inhibitory effect of Malvidin on TNF-?-induced inflammatory response in endothelial cells." dans: European journal of pharmacology, Vol. 723, pp. 67-72, (2014) (PubMed).

Asgary, Keshvari, Afshani, Amiri, Laher, Javanmard: "Effect of fresh orange juice intake on physiological characteristics in healthy volunteers." dans: ISRN nutrition, Vol. 2014, pp. 405867, (2014) (PubMed).

Asgary, Kelishadi, Rafieian-Kopaei, Najafi, Najafi, Sahebkar: "Investigation of the lipid-modifying and antiinflammatory effects of Cornus mas L. supplementation on dyslipidemic children and adolescents." dans: Pediatric cardiology, Vol. 34, Issue 7, pp. 1729-35, (2013) (PubMed).

Détail du antigène

Antigène: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Autre désignation: VCAM1

Sujet:

Background: Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9KDa. As a result of glycosylation, the molecular mass of 120KDa is revealed by SDS-PAGE.

Gene Full Name: vascular cell adhesion molecule 1

ID gène: 22329

UniProt: P29533

Pathways: Carbohydrate Homeostasis