VEGF Kit ELISA

N° du produit ABIN411371

Détail du produit VEGF Kit ELISA

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Épitope: AA 27-190

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse VEGF

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: sf21,A27-R190

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: sf21
Immunogen sequence: A27-R190

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Commentaires:

Sequence similarities: Belongs to the PDGF/VEGF growth factor family.

Tissue Specificity: In developing embryos, expressed mainly in the choroid plexus, paraventricular neuroepithelium, placenta and kidney glomeruli. Also found in bronchial epithelium, adrenal gland and in seminiferous tubules of testis. High expression of VEGF continues in kidney glomeruli and choroid plexus in adults.

Plaque: Pre-coated

Protocole: mouse VEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF has been precoated onto 96-well plates. Standards (sf21,A27-R190) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGF amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.3pg/mL, 15.6pg/mL mouse VEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse VEGF standard solution and each sample is measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 126, Standard deviation: 4.41, CV(%): 3.5
• Sample 2: n=16, Mean(pg/ml): 341, Standard deviation: 13.64, CV(%): 4
• Sample 3: n=16, Mean(pg/ml): 580, Standard deviation: 28.42, CV(%): 4.9,
• Sample 1: n=24, Mean(pg/ml): 212, Standard deviation: 10.6, CV(%): 5
• Sample 2: n=24, Mean(pg/ml): 475, Standard deviation: 30.4, CV(%): 6.4
• Sample 3: n=24, Mean(pg/ml): 624, Standard deviation: 46.8, CV(%): 7.5

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour VEGF Kit ELISA (ABIN411371)

Chen, Qiao, Tang, Chen, Liu, Yang, Wei, Zhang, Cheng, Du, Jiang, Hu, Hua: "Modulation of Salmonella Tumor-Colonization and Intratumoral Anti-angiogenesis by Triptolide and Its Mechanism." dans: Theranostics, Vol. 7, Issue 8, pp. 2250-2260, (2018) (PubMed).

Li, Ding, Ling, Wu, Meng, Li, Zhang: "Metformin attenuates motility, contraction, and fibrogenic response of hepatic stellate cells in vivo and in vitro by activating AMP-activated protein kinase." dans: World journal of gastroenterology, Vol. 24, Issue 7, pp. 819-832, (2018) (PubMed).

Zou, Wang, Liu, Bai, Wang, Liu, Zhang, Xiao, Wang: "Nucleolin mediated pro-angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post-transcriptional regulation of VEGF-A and MMP-9." dans: Journal of cellular and molecular medicine, Vol. 22, Issue 5, pp. 2692-2705, (2018) (PubMed).

Cheng, Chen, Su, Su, Hou: "A novel dressing seeded with embryonic artery CD133+ cells and loaded with the Sirt1 agonist SRT1720 accelerates the healing of diabetic ischemic ulcers." dans: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 5243-5250, (2018) (PubMed).

Wang, Liu, Chen, Chen: "Physicochemical characterization of the oolong tea polysaccharides with high molecular weight and their synergistic effects in combination with polyphenols on hepatocellular carcinoma." dans: Biomedicine & pharmacotherapy, Vol. 90, pp. 160-170, (2017) (PubMed).

Li, Dai, Shen, Gao: "Induced migration of endothelial cells into 3D scaffolds by chemoattractants secreted by pro-inflammatory macrophages in situ." dans: Regenerative biomaterials, Vol. 4, Issue 3, pp. 139-148, (2017) (PubMed).

Li, Wang, Wang, Zhang, Wu, Yang, Ji, Li, Wang: "Occludin downregulation in high glucose is regulated by SSTR2 via the VEGF/NRP1/Akt signaling pathway in RF/6A cells." dans: Experimental and therapeutic medicine, Vol. 14, Issue 2, pp. 1732-1738, (2017) (PubMed).

Sun, Chen, Zhang, Shen, Wang, Wang, Liu, Liu, Liu, Liu, Jin: "β2-Adrenergic Receptor-Mediated HIF-1α Upregulation Mediates Blood Brain Barrier Damage in Acute Cerebral Ischemia." dans: Frontiers in molecular neuroscience, Vol. 10, pp. 257, (2017) (PubMed).

Abdel-Gawad, Hassan, Awwad: "Efficiency of calcium phosphate composite nanoparticles in targeting Ehrlich carcinoma cells transplanted in mice." dans: Journal of advanced research, Vol. 7, Issue 1, pp. 143-54, (2016) (PubMed).

Sancakdar, Guven, Uysal, Deveci, Gültürk: "Important of Angiopoietic System in Evaluation of Endothelial Damage in Children with Crimean-Congo Hemorrhagic Fever." dans: The Pediatric infectious disease journal, Vol. 34, Issue 8, pp. e200-5, (2015) (PubMed).

Jiang, Han, Li, Yang, Liu: "Carboxymethyl chitosan represses tumor angiogenesis in vitro and in vivo." dans: Carbohydrate polymers, Vol. 129, pp. 1-8, (2015) (PubMed).

Jiang, Han, Li, Li, Yang, Liu: "Preparation and anti-tumor metastasis of carboxymethyl chitosan." dans: Carbohydrate polymers, Vol. 125, pp. 53-60, (2015) (PubMed).

Das, Jargar, Saha, Yendigeri, Singh: "?-tocopherol supplementation prevents lead acetate and hypoxia-induced hepatic dysfunction." dans: Indian journal of pharmacology, Vol. 47, Issue 3, pp. 285-91, (2015) (PubMed).

Fu, Chen, Cheng, Wang: "Hypoxia-inducible factor-1? protects cervical carcinoma cells from apoptosis induced by radiation via modulation of vascular endothelial growth factor and p53 under hypoxia." dans: Medical science monitor : international medical journal of experimental and clinical research, Vol. 21, pp. 318-25, (2015) (PubMed).

Fan, Zhang, Xu, Ke, Ding, Wang, Liu, Ning, Deng, He: "Serum level of vascular endothelial growth factor decreased in chronic ketamine abusers." dans: Drug and alcohol dependence, Vol. 152, pp. 57-61, (2015) (PubMed).

Zhang, Chen, Fan, Wang, Zhu: "Overexpression of VEGF183 promotes murine breast cancer cell proliferation in vitro and induces dilated intratumoral microvessels." dans: Tumour biology, Vol. 36, Issue 5, pp. 3871-80, (2015) (PubMed).

Chen, Xu, Li, Du, Chen, Liu, Peng, Wang, Song: "Epigallocatechin-3-gallate attenuates lipopolysaccharide-induced mastitis in rats via suppressing MAPK mediated inflammatory responses and oxidative stress." dans: International immunopharmacology, Vol. 26, Issue 1, pp. 147-52, (2015) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." dans: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Wang, Zeng, Wang, Liu, Xiao, Xiang: "Evaluating the potential bioactivity of a novel compound ER1626." dans: PLoS ONE, Vol. 9, Issue 1, pp. e86509, (2014) (PubMed).

Ge, Ding, Zhang, Li, Li: "Effect of angiogenesis inhibitor SU6668 in combination with 5-Fu on liver metastasis from transplantation tumors of human colorectal cancer in nude mice." dans: International journal of clinical and experimental medicine, Vol. 7, Issue 10, pp. 3578-82, (2014) (PubMed).

Détail du antigène

Antigène: VEGF (Vascular Endothelial Growth Factor (VEGF))

Abstract: VEGF Produits

Synonymes: MVCD1 Kit ELISA, VEGF Kit ELISA, VPF Kit ELISA, Vegf Kit ELISA, Vegf120 Kit ELISA, Vegf164 Kit ELISA, Vegf188 Kit ELISA, Vpf Kit ELISA, VEGF-A Kit ELISA, VEGF164 Kit ELISA, eVEGF120 Kit ELISA, eVEGF164 Kit ELISA, vegf-a Kit ELISA, vegf Kit ELISA, vascular endothelial growth factor A Kit ELISA, vascular endothelial growth factor A L homeolog Kit ELISA, vascular endothelial growth factor Kit ELISA, vascular endothelial growth factor precursor Kit ELISA, VEGFA Kit ELISA, Vegfa Kit ELISA, vegfa.L Kit ELISA, VEGF Kit ELISA, vegf Kit ELISA

Sujet:

Protein Function: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis (By similarity). .

Background: Vascular permeability factor/vascular endothelial growth factor(VPF/VEGF), a potent cytokine expressed by most malignant tumors, has critical roles in vasculogenesis and both physiological and pathological angiogenesis. VEGF produced by tumor cells potently stimulates endothelial cell proliferation and angiogenesis and plays a key role in the pathophysiology of several neoplasias. VEGF may also play a pivotal role in mediating the development and progression of diabetic retinopathy. VEGF, a major regulator of angiogenesis, binds to two receptor tyrosine kinases, KDR/Flk-1 and Flt-1.The VEGF gene is mapped by fluorescence in situ hybridization to chromosome 6p12. The standard product used in this kit is recombinant mouse VEGF164, a 42KDa dimer.

Synonyms: Vascular endothelial growth factor A,VEGF-A,Vascular permeability factor,VPF,Vegfa,Vegf,

Full Gene Name: Vascular endothelial growth factor A

Cellular Localisation: Isoform VEGF-1: Secreted.

ID gène: 22339

UniProt: Q00731