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ADIPOQ Kit ELISA

ADIPOQ Reactivité: Humain AA 19-244 Colorimetric Sandwich ELISA 1.56-100 ng/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate, Urine
N° du produit ABIN411384
  • Antigène Voir toutes ADIPOQ Kits ELISA
    ADIPOQ (Adiponectin (ADIPOQ))
    Épitope
    AA 19-244
    Reactivité
    • 9
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56-100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Adiponectin
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: E19-N244
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <60pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: E19-N244
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    human Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(NSO, E19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Adiponectin amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.12 ng/mL, 1.56 ng/mL human Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Adiponectin standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 13.8, Standard deviation: 0.39, CV(%): 2.8
    • Sample 2: n=16, Mean(ng/ml): 37.2, Standard deviation: 1.34, CV(%): 3.6
    • Sample 3: n=16, Mean(ng/ml): 67.3, Standard deviation: 3.03, CV(%): 4.5,
    • Sample 1: n=24, Mean(ng/ml): 13.1, Standard deviation: 0.77, CV(%): 5.9
    • Sample 2: n=24, Mean(ng/ml): 36.8, Standard deviation: 2.50, CV(%): 6.8
    • Sample 3: n=24, Mean(ng/ml): 65.7, Standard deviation: 4.66, CV(%): 7.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Buturak, Değirmencioğlu, Bayrak, Kırış, Karakurt, Demir, Sürgit, Ertürk: "Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study." dans: Anatolian journal of cardiology, Vol. 16, Issue 12, pp. 940-946, (2018) (PubMed).

    Altay, Seçil, Demir, Atik, Akıncı, Özdemir Kutbay, Keskin Temeloğlu, Yıldırım Şimşir, Özışık, Demir, Eren, Tuna, Aytaç, Onay, Akıncı: "Determining residual adipose tissue characteristics with MRI in patients with various subtypes of lipodystrophy." dans: Diagnostic and interventional radiology (Ankara, Turkey), Vol. 23, Issue 6, pp. 428-434, (2018) (PubMed).

    Küme, Acar, Tuhan, Çatlı, Anık, Gürsoy Çalan, Böber, Abacı: "The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity." dans: Journal of clinical research in pediatric endocrinology, Vol. 9, Issue 1, pp. 31-38, (2017) (PubMed).

    Koh, Park: "Responses of inflammatory cytokines following moderate intensity walking exercise in overweight or obese individuals." dans: Journal of exercise rehabilitation, Vol. 13, Issue 4, pp. 472-476, (2017) (PubMed).

    Mohammadi, Paknahad: "Adiponectin Concentration in Gestational Diabetic Women: a Case-Control Study." dans: Clinical nutrition research, Vol. 6, Issue 4, pp. 267-276, (2017) (PubMed).

    Naini, Vahdat, Hedaiati, Shahzeidi, Pezeshki, Nasri et al.: "The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical ..." dans: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 21, pp. 1, (2016) (PubMed).

    Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

    Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." dans: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

    Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." dans: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

    Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." dans: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

    Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." dans: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

    Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." dans: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).

  • Antigène Voir toutes ADIPOQ Kits ELISA
    ADIPOQ (Adiponectin (ADIPOQ))
    Autre désignation
    ADIPOQ (ADIPOQ Produits)
    Synonymes
    ADP Kit ELISA, DCAF9 Kit ELISA, 6720489L24Rik Kit ELISA, 9330209L04Rik Kit ELISA, Adp Kit ELISA, Mamdc1 Kit ELISA, RNSVSP4U Kit ELISA, SVSIV1 Kit ELISA, Svp4 Kit ELISA, ACDC Kit ELISA, ACRP30 Kit ELISA, ADIPQTL1 Kit ELISA, ADPN Kit ELISA, APM-1 Kit ELISA, APM1 Kit ELISA, GBP28 Kit ELISA, apM-1 Kit ELISA, 30kDa Kit ELISA, APN Kit ELISA, Acdc Kit ELISA, Acrp30 Kit ELISA, Ad Kit ELISA, adipo Kit ELISA, apM1 Kit ELISA, ADN Kit ELISA, acrpl Kit ELISA, adipo-b Kit ELISA, adipoql Kit ELISA, zgc:153584 Kit ELISA, adipoq Kit ELISA, WD and tetratricopeptide repeats 1 Kit ELISA, MAM domain containing glycosylphosphatidylinositol anchor 2 Kit ELISA, seminal vesicle secretory protein 4 Kit ELISA, adiponectin, C1Q and collagen domain containing Kit ELISA, adiponectin, C1Q and collagen domain containing, b Kit ELISA, adiponectin, C1Q and collagen domain containing L homeolog Kit ELISA, WDTC1 Kit ELISA, Mdga2 Kit ELISA, Svs4 Kit ELISA, ADIPOQ Kit ELISA, Adipoq Kit ELISA, adipoqb Kit ELISA, adipoq.L Kit ELISA
    Sujet

    Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.

    Synonyms: Adiponectin ,Adiponectin, C1Q and collagen domain containing ,cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ,ADIPOQ ,hCG_1784052 ,

    Full Gene Name: adiponectin, C1Q and collagen domain containing

    ID gène
    9370
    UniProt
    A8K660
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