Vip Kit ELISA

N° du produit ABIN414396

Le kit ELISA Vip (ABIN414396) Humain est un kit ELISA Colorimetric conçu pour quantifier Humain Vip. Ce kit ELISA a été cité dans 12 publications.

Aperçu rapide pour Vip Kit ELISA (ABIN414396)

Antigène: Vip (Vasoactive Intestinal Peptide (Vip))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 6.17 pg/mL - 500 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Détail du produit Vip Kit ELISA

Seuil minimal de détection: 6.17 pg/mL

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of VIP in Serum,Plasma,Tissue Homogenate,Cell Lysate,Cell Culture Supernatant,Biological Fluids

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Vasoactive Intestinal Peptide (VIP).
No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Vasoactive Intestinal Peptide (VIP) and analogues was observed.

Sensibilité: < 2.63 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vasoactive Intestinal Peptide (VIP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour Vip Kit ELISA (ABIN414396)

Haririan, Andrukhov, Böttcher, Pablik, Wimmer, Moritz, Rausch-Fan: "Salivary neuropeptides, stress, and periodontitis." dans: Journal of periodontology, Vol. 89, Issue 1, pp. 9-18, (2018) (PubMed).

Ge, Duan, Yang, Zhang, Zhang, Wang, Yang, Sun, Zhang, Su, Zhu, Zhou, Liu, Shi, Yu, Yang, Chang, Zhang, Wu, Chen: "Home-Based Transcutaneous Neuromodulation Improved Constipation via Modulating Gastrointestinal Hormones and Bile Acids." dans: Evidence-based complementary and alternative medicine : eCAM, Vol. 2018, pp. 2086163, (2018) (PubMed).

Peng, Liu, Hu, Yan, Gao, Wu: "Using the "target constituent removal combined with bioactivity assay" strategy to investigate the optimum arecoline content in charred areca nut." dans: Scientific reports, Vol. 7, pp. 40278, (2017) (PubMed).

McDonald, Smith, Smith, Changli Xue, Golianu, Cripps: "Effect of acupuncture on house dust mite specific IgE, substance P, and symptoms in persistent allergic rhinitis." dans: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 116, Issue 6, pp. 497-505, (2016) (PubMed).

Riesco, Cernuda-Morollón, Martínez-Camblor, Pérez-Alvarez, Verano, García-Cabo, Serrano-Pertierra, Pascual: "Relationship between serum levels of VIP, but not of CGRP, and cranial autonomic parasympathetic symptoms: A study in chronic migraine patients." dans: Cephalalgia : an international journal of headache, (2016) (PubMed).

Cernuda-Morollón, Riesco, Martínez-Camblor, Serrano-Pertierra, García-Cabo, Pascual: "No Change in Interictal PACAP Levels in Peripheral Blood in Women With Chronic Migraine." dans: Headache, Vol. 56, Issue 9, pp. 1448-1454, (2016) (PubMed).

Cernuda-Morollón, Martínez-Camblor, Alvarez, Larrosa, Ramón, Pascual: "Increased VIP levels in peripheral blood outside migraine attacks as a potential biomarker of cranial parasympathetic activation in chronic migraine." dans: Cephalalgia : an international journal of headache, Vol. 35, Issue 4, pp. 310-6, (2015) (PubMed).

Ul Kabir, Samad, Ahmed, Jahan, Akhter, Tasnim, Hasan, Sayfe, Hannan et al.: "Aqueous fraction of beta vulgaris ameliorates hyperglycemia in diabetic mice due to enhanced glucose stimulated insulin secretion, mediated by acetylcholine and GLP-1, and elevated glucose uptake via ..." dans: PLoS ONE, Vol. 10, Issue 2, pp. e0116546, (2015) (PubMed).

Xiong, Wang, Li, Tian, Zheng, Huang: "Clinical study of electro-acupuncture treatment with different intensities for functional constipation patients." dans: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban, Vol. 34, Issue 5, pp. 775-81, (2014) (PubMed).

Cernuda-Morollón, Martínez-Camblor, Ramón, Larrosa, Serrano-Pertierra, Pascual: "CGRP and VIP levels as predictors of efficacy of Onabotulinumtoxin type A in chronic migraine." dans: Headache, Vol. 54, Issue 6, pp. 987-95, (2014) (PubMed).

Corrêa, da Costa Rocha, de Sousa, do Carmo Pereira Nunes, Gollob, Dutra, da Silva Menezes: "Low levels of vasoactive intestinal peptide are associated with Chagas disease cardiomyopathy." dans: Human immunology, Vol. 74, Issue 10, pp. 1375-81, (2013) (PubMed).

Zhang, Zhang, Liu, Xiong, Ni, Hui, He, Pu: "VIP gene variants related to idiopathic pulmonary arterial hypertension in Chinese population." dans: Clinical genetics, Vol. 75, Issue 6, pp. 544-9, (2009) (PubMed).

Détail du antigène

Antigène: Vip (Vasoactive Intestinal Peptide (Vip))

Autre désignation: VIP

Sujet: PHM27, Intestinal peptide PHV-42, Peptide histidine valine 42, Peptide histidine methioninamide 27

UniProt: P01282

Pathways: Hormone Activity, cAMP Metabolic Process