MMP13 Kit ELISA

N° du produit ABIN415612

Kit ELISA MMP13 Souris, Colorimetric test pour la quantification de Souris MMP13 et a été mentionné dans 13+ publications.

Aperçu rapide pour MMP13 Kit ELISA (ABIN415612)

Antigène: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Détail du produit MMP13 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP13 in mouse serum, plasma.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Analytical Method: Quantitative

Specificité:

This assay has high sensitivity and excellent specificity for detection of Matrix Metalloproteinase 13 (MMP13).
No significant cross-reactivity or interference between Matrix Metalloproteinase 13 (MMP13) and analogues was observed.

Réactivité croisée (Details): No significant cross-reactivity or interference between Matrix Metalloproteinase 13 (MMP13) and analogues was observed.

Sensibilité: < 6.3 pg/mL

Ingrédients:

• Pre-coated, 96-well strip plate
• Plate sealer
• Standard
• Standard Diluent
• Detection Reagent A
• Assay Diluent A
• Detection Reagent B
• Assay Diluent B
• TMB Substrate
• Stop Solution
• Wash Buffer (30X)
• Instruction manual

Matériel non inclus:

• Microplate reader with 450 nm filter.
• Precision single or multi-channel pipettes and disposable tips.
• Eppendorf Tubes for diluting samples.
• Deionized or distilled water.
• Absorbent paper for blotting the microtiter plate.
• Container for Wash Solution

Information d'application

Indications d'application:

• Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
• The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
• Kits from different batches may be a little different in detection range, sensitivity and color developing time.
• Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
• Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
• There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
• Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
• Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
• Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
• Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Précision du teste:

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Matrix Metalloproteinase 13 (MMP13) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Conseil sur la manipulation: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Stock: 4 °C

Stockage commentaire:

• For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
• For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
  Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
• For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.

Date de péremption: 6 months

Références pour MMP13 Kit ELISA (ABIN415612)

Momma, Onodera, Homan, Matsubara, Sasazawa, Furukawa, Matsuoka, Yamashita, Iwasaki: "Coordinated existence of multiple gangliosides is required for cartilage metabolism." dans: Osteoarthritis and cartilage, Vol. 27, Issue 2, pp. 314-325, (2020) (PubMed).

Chen, Che, Han, Lu, Wang, Liang, Hou, Wei, Wei, Li: "MicroRNA-1 promotes cartilage matrix synthesis and regulates chondrocyte differentiation via post-transcriptional suppression of Ihh expression." dans: Molecular medicine reports, Vol. 22, Issue 3, pp. 2404-2414, (2020) (PubMed).

Pitrez, Estronca, Monteiro, Colell, Vazão, Santinha, Harhouri, Thornton, Navarro, Egesipe, Carvalho, Dos Santos, Lévy, Smith, de Magalhães, Ori, Bernardo, De Sandre-Giovannoli, Nissan, Rosell et al.: "Vulnerability of progeroid smooth muscle cells to biomechanical forces is mediated by MMP13. ..." dans: Nature communications, Vol. 11, Issue 1, pp. 4110, (2020) (PubMed).

Xie, Wen, Yan, Wang, Wang, Chen, Li, Xu, Zhong, Shen, Chu: "Toxoplasma gondii GRA15II effector-induced M1 cells ameliorate liver fibrosis in mice infected with Schistosomiasis japonica." dans: Cellular & molecular immunology, (2016) (PubMed).

Misawa, Tanaka, Saito, Nabeshima, Yao, Yamauchi, Abe, Yamamoto, Furukawa: "Protective effects of Aloe sterols against UVB-induced photoaging in hairless mice." dans: Photodermatology, photoimmunology & photomedicine, Vol. 33, Issue 2, pp. 101-111, (2016) (PubMed).

Goto, Ishihara, Kikuchi, Izawa, Ozeki, Okabe, Kamiya, Ozawa, Mizutani, Yamamoto, Mogi, Nakata, Maeda, Noguchi, Mitani: "Interleukin-1 Receptor Antagonist Has a Novel Function in the Regulation of Matrix Metalloproteinase-13 Expression." dans: PLoS ONE, Vol. 10, Issue 10, pp. e0140942, (2015) (PubMed).

Du, Wu, Qing, Wang, Liang, Yu, Tang: "Systemic and flap inflammatory response associates with thrombosis in flap venous crisis." dans: Inflammation, Vol. 38, Issue 1, pp. 298-304, (2015) (PubMed).

Sasazawa, Onodera, Yamashita, Seito, Tsukuda, Fujitani, Shinohara, Iwasaki: "Depletion of gangliosides enhances cartilage degradation in mice." dans: Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society, Vol. 22, Issue 2, pp. 313-22, (2014) (PubMed).

Lenglet, Montecucco, Mach, Schaller, Gasche, Copin: "Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke." dans: Thrombosis and haemostasis, Vol. 112, Issue 2, pp. 363-78, (2014) (PubMed).

Seito, Yamashita, Tsukuda, Matsui, Urita, Onodera, Mizutani, Haga, Fujitani, Shinohara, Minami, Iwasaki: "Interruption of glycosphingolipid synthesis enhances osteoarthritis development in mice." dans: Arthritis and rheumatism, Vol. 64, Issue 8, pp. 2579-88, (2012) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Prestipino, Calatroni, Campo: "Hyaluronan in part mediates IL-1beta-induced inflammation in mouse chondrocytes by up-regulating CD44 receptors." dans: Gene, Vol. 494, Issue 1, pp. 24-35, (2012) (PubMed).

Campo, Avenoso, DAscola, Scuruchi, Prestipino, Nastasi, Calatroni, Campo: "Adenosine A2A receptor activation and hyaluronan fragment inhibition reduce inflammation in mouse articular chondrocytes stimulated with interleukin-1?." dans: The FEBS journal, Vol. 279, Issue 12, pp. 2120-33, (2012) (PubMed).

Campo, Avenoso, Nastasi, Micali, Prestipino, Vaccaro, DAscola, Calatroni, Campo: "Hyaluronan reduces inflammation in experimental arthritis by modulating TLR-2 and TLR-4 cartilage expression." dans: Biochimica et biophysica acta, Vol. 1812, Issue 9, pp. 1170-81, (2011) (PubMed).

Détail du antigène

Antigène: MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))

Autre désignation: MMP13

Sujet: CLG3, Collagenase 3

UniProt: P33435