Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) Kit ELISA

N° du produit ABIN454021

Kit ELISA Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) Humain, Colorimetric test pour la quantification de Humain Anti-Alpha2 Adrenergic Receptor Antibodies (IgG).

Aperçu rapide pour Anti-Alpha2 Adrenergic Receptor Antibodies (IgG) Kit ELISA (ABIN454021)

Antigène: Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Application: ELISA

Détail du produit

Fonction: The anti-alpha2-adrenergic-receptor-antibody-EIA is an antibody screening test. The alpha2-adrenergic-receptor has been pre-coated onto a microtiter plate. During the first incubation the anti-alpha2-adrenergic-receptor-antibodies of the samples are immobilised on the plate. The auto-antibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-alpha2-adrenergic-receptor-antibodies.

Analytical Method: Quantitative

Information d'application

Plaque: Pre-coated

Procédure de l'essai:

Incubation of samples/ standards/ controls: 100 µL 120 min, 4°C
Wash Incubation of detection antibody: 100 µL 1 hr, room temperature
Wash Substrate incubation: 100 µL/well 20 min, room temperature
Add Stopp solution: 100 µL/well
Read at 450nm

Précision du teste: Intraassay-Precision:9,12% Interassay-Precision:13.44%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Anti-Alpha2 Adrenergic Receptor Antibodies (IgG)

Classe de substances: Antibody

Sujet: The Alpha-2 adrenergic receptor is a G protein-coupled receptor. ß receptors have the subtypes ß1, ß2 and ß3. Agonist binding causes a rise in the intracellular concentration of the second messenger cAMP. The anti-alpha2-adrenergic-receptor-antibody-EIA is designed for the determination of Antibodies (IgG) against the Alpha2-adrenergic-receptor in serum and plasma.