IgA Kit ELISA

N° du produit ABIN454025

Détail du produit IgA Kit ELISA

Antigène: IgA

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Seuil minimal de détection: 156 ng/mL

Application: ELISA

Fonction: The IgA(rat)-ELISA is designed for the quantitative determination of rat IgA in complex samples (serum or other biological samples).

Type d'échantillon: Serum

Analytical Method: Quantitative

Attributs du produit: The determination of rat IgA is carried out as direct sandwich ELISA.

Information d'application

Plaque: Pre-coated

Protocole: An antibody specific for rat IgA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgA present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgA concentration.

Procédure de l'essai:

Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm

Précision du teste: Intraassay precision (CV):(n=10)at 43 ng/ml: 3.8%at 99 ng/ml: 6.9%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IgA

Abstract: IgA Produits

Synonymes: MB-1 Kit ELISA, IG-alpha Kit ELISA, IGA Kit ELISA, IgA Kit ELISA, Igh-2 Kit ELISA, CD79a molecule Kit ELISA, immunoglobulin heavy constant alpha Kit ELISA, CD79A Kit ELISA, Igha Kit ELISA

Classe de substances: Antibody