IgG Kit ELISA
Aperçu rapide pour IgG Kit ELISA (ABIN454027)
Antigène
Voir toutes IgG Kits ELISAReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
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Seuil minimal de détection
- 156 ng/mL
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Fonction
- The IgG(pig)-ELISA is designed for the quantitative determination of pig IgG in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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Attributs du produit
- The determination of pig IgG is carried out as direct sandwich ELISA.
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IgG ELISA Kit
Reactivité: Lapin Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Rabbit TrueBlot® Set (with IP beads)WB, IP Reactivité: Lapin
Mouse TrueBlot® Set (with IP beads)WB, IP Reactivité: Souris
IgG ELISA KitReactivité: Souris Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivité: Porc Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivité: Souris Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG ELISA KitReactivité: Humain Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivité: Poulet Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
IgG ELISA KitReactivité: Boeuf (Vache) Colorimetric Competition ELISA 1.23 μg/mL - 100 μg/mL Plasma, Serum
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Plaque
- Pre-coated
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Protocole
- An antibody specific for pig IgG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgG present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgG concentration.
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Procédure de l'essai
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Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm -
Précision du teste
- Intraassay precision (CV): (n=10) at 57 ng/ml: 5.3% at 218 ng/ml: 2.7% at 580 ng/ml: 4.7%
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IgG
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Classe de substances
- Antibody
Antigène Voir toutes IgG Kits ELISA
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