IgM Kit ELISA

N° du produit ABIN454032

Détail du produit IgM Kit ELISA

Antigène: IgM

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Seuil minimal de détection: 312.5 ng/mL

Application: ELISA

Fonction: The IgM(rat)-ELISA is designed for the quantitative determination of rat IgM in complex samples (serum or other biological samples).

Type d'échantillon: Serum

Analytical Method: Quantitative

Attributs du produit: The determination of rat IgM is carried out as direct sandwich ELISA.

Information d'application

Plaque: Pre-coated

Protocole: An antibody specific for rat IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.

Procédure de l'essai:

Incubation of samples/standards: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Incubation of detection antibody: 100 µL/well 1 hr with shaking or 2 hrs without shaking
Wash Substrate incubation: 100 µL/well 10 min, room temperature
Add stop solution: 100 µL/well
Read at 450 nm

Précision du teste: Intraassay precision (CV):(n=10)at 70 ng/ml: 7.6%at 250 ng/ml: 6.8%

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: IgM

Abstract: IgM Produits

Synonymes: IgM constant region Kit ELISA, IGM Kit ELISA

Classe de substances: Antibody