Aquaporin 4 Kit ELISA

N° du produit ABIN454459

Kit ELISA Aquaporin 4 Souris, Colorimetric test pour la quantification de Souris Aquaporin 4.

Aperçu rapide pour Aquaporin 4 Kit ELISA (ABIN454459)

Antigène: Aquaporin 4 (AQP4)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Application: ELISA

Type d'échantillon: Tissue Homogenate

Détail du produit Aquaporin 4 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse AQP-4 concentrations in tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse AQP-4.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Aquaporin-4,AQP-4,Mercurial-insensitive water channel,MIWC,WCH4,Aqp4

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of AQP-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: Aquaporin 4 (AQP4)

Autre désignation: Aqp4

Sujet: Aquaporin-4 (AQP4), is a channel protein that allows water to move in and out of cells. Aquaporin-4 is located in many tissues, including the basolateral membrane of the renal cortical and medullary principal cell, as well as in the brain.Aquaporin-4 is the first specific molecule to be defined as a target for the autoimmune response in any form of multiple sclerosis. Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.

Pathways: Sensory Perception of Sound