Aquaporin 4 Kit ELISA
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- Antigène Voir toutes Aquaporin 4 (AQP4) Kits ELISA
- Aquaporin 4 (AQP4)
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.156-10 ng/mL
- Seuil minimal de détection
- 0.156 ng/mL
- Application
- ELISA
- Fonction
- This immunoassay kit allows for the in vitro quantitative determination of mouse AQP-4 concentrations in tissue homogenates and other biological fluids.
- Type d'échantillon
- Tissue Homogenate
- Analytical Method
- Quantitative
- Specificité
- This assay recognizes recombinant and natural mouse AQP-4.
- Réactivité croisée (Details)
- No significant cross-reactivity or interference was observed.
- Sensibilité
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< 0.039 ng/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero. - Attributs du produit
- Mus musculus,Mouse,Aquaporin-4,AQP-4,Mercurial-insensitive water channel,MIWC,WCH4,Aqp4
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- Discover our best selling AQP4 Kit ELISA
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- Discover our top product AQP4 Kit ELISA
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- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
- The microtiter plate provided in this kit has been pre-coated with an antibody specific to AQP-4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AQP-4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AQP-4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The concentration of AQP-4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Stock
- 4 °C/-20 °C
- Stockage commentaire
- The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
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- Antigène Voir toutes Aquaporin 4 (AQP4) Kits ELISA
- Aquaporin 4 (AQP4)
- Autre désignation
- Aqp4 (AQP4 Produits)
- Synonymes
- zgc:100922 Kit ELISA, si:ch211-192k9.1 Kit ELISA, AQP4 Kit ELISA, LOC100232616 Kit ELISA, HMIWC2 Kit ELISA, MIWC Kit ELISA, WCH4 Kit ELISA, AQP-4 Kit ELISA, AQP4.M23 Kit ELISA, MIWC2 Kit ELISA, Miwc Kit ELISA, aquaporin-4 Kit ELISA, aquaporin 4 Kit ELISA, aquaporin 4 L homeolog Kit ELISA, aqp4 Kit ELISA, AQP4 Kit ELISA, aqp4.L Kit ELISA, Aqp4 Kit ELISA
- Sujet
- Aquaporin-4 (AQP4), is a channel protein that allows water to move in and out of cells. Aquaporin-4 is located in many tissues, including the basolateral membrane of the renal cortical and medullary principal cell, as well as in the brain.Aquaporin-4 is the first specific molecule to be defined as a target for the autoimmune response in any form of multiple sclerosis. Aquaporin 4 is found in the basolateral cell membrane of principal collecting duct cells and provide a pathway for water to exit these cells.
- Pathways
- Sensory Perception of Sound
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