HMGB4 Kit ELISA

N° du produit ABIN454716

Ce kit ELISA Humain HMGB4 est un kit ELISA Colorimetric conçu pour quantifier Humain HMGB4.

Aperçu rapide pour HMGB4 Kit ELISA (ABIN454716)

Antigène: HMGB4 (High Mobility Group Box 4 (HMGB4))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit HMGB4 Kit ELISA

Seuil minimal de détection: 0.156 ng/mL

Fonction: This immunoassay kit allows for the in vitro quantitative determination of human chondroitin sulfatase,CS concentrations in cell culture supernates, serum, plasma and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human CS.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 0.312 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Homo sapiens,Human,High mobility group protein B4,HMGB4

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CS in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: HMGB4 (High Mobility Group Box 4 (HMGB4))

Autre désignation: HMGB4

Sujet: In enzymology, a chondroitin sulfatase is an enzyme that catalyzes the chemical reaction of cleaving off the 6-sulfate groups of the N-acetyl-D-galactosamine 6-sulfate units of the macromolecule chondroitin sulfate and, similarly, of the D-galactose 6-sulfate units of the macromolecule keratan sulfate.This enzyme belongs to the family of hydrolases, specifically those acting on sulfuric ester bonds.