LDHD Kit ELISA

N° du produit ABIN454979

Détail du produit LDHD Kit ELISA

Antigène: LDHD (Lactate Dehydrogenase D (LDHD))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of Mouse D-LDH, concentrations in serum, tissue homogenates and other biological fluids.

Type d'échantillon: Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural Mouse D-LDH.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Probable D-lactate dehydrogenase, mitochondrial,DLD,Lactate dehydrogenase D,Ldhd,1.1.2.4

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to D-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for D-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain D-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of D-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: LDHD (Lactate Dehydrogenase D (LDHD))

Autre désignation: Ldhd (LDHD Produits)

Synonymes: DLD Kit ELISA, 4733401P21Rik Kit ELISA, D8Bwg1320e Kit ELISA, Ac2-202 Kit ELISA, lactate dehydrogenase D Kit ELISA, LDHD Kit ELISA, ldhd Kit ELISA, Ldhd Kit ELISA

Pathways: Ribonucleoside Biosynthetic Process