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FTH1 Kit ELISA

FTH1 Reactivité: Humain Colorimetric Sandwich ELISA 1.56-100 ng/mL Plasma, Serum, Urine
N° du produit ABIN455101
  • Antigène Voir toutes FTH1 Kits ELISA
    FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
    Reactivité
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    1.56-100 ng/mL
    Seuil minimal de détection
    1.56 ng/mL
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the specific measurement of Human Ferritin concentrations in urine ,serum and plasma.
    Type d'échantillon
    Urine, Serum, Plasma
    Analytical Method
    Quantitative
    Specificité
    This assay recognizes natural Human Ferritin.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference was observed.
    Sensibilité
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Attributs du produit
    Homo sapiens,Human,Ferritin heavy chain,Ferritin H subunit,Cell proliferation-inducing gene 15 protein,FTH1,FTH,FTHL6,OK/SW-cl.84,PIG15,1.16.3.1
    Ingrédients
    Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A 1 x120µl Detection Reagent B (1x120µl), Wash Buffer 1 x 30ml 2 (25 x concentrate) Substrate (1x10ml), Stop Solution (1x10ml)
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Ferritin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Ferritin present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for Ferritin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Ferritin bound in the initial step. The color development is stopped and the intensity of the color is measured.
    Préparation des réactifs

    Bring all reagents to room temperature before use. Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 500 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0 mL of Sample Diluent. This reconstitution produces a stock solution of 100 ng/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (100 ng/mL). The Sample Diluent serves as the zero standard (0 ng/mL). Detection Reagent A and B - Dilute to the working concentration specified on the vial label using Assay Diluent A and B (1:100), respectively.

    Prélèvement de l'échantillon
    Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2 - 8 °C within 30 minutes of collection. Store samples at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Urine - Aseptically collect the first urine of the day (mid-stream), voided directly into a sterile container. Centrifuge to remove particulate matter, assay immediately or aliquot and store at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Note: Citrate plasma has not been validated for use in this assay.
    Procédure de l'essai

    Allow all reagents to reach room temperature. Arrange and label required number of strips.
    1. Prepare all reagents, working standards and samples as directed in the previous sections. 3
    2. Add 100 uL of Standard, Control, or sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37 °C.
    3. Remove the liquid of each well, don’t wash.
    4. Add 100 uL of Detection Reagent A to each well. Incubate for 1 hour at 37°C. Detection Reagent A may appear cloudy. Warm to room temperature and mix gently until solution appears uniform.
    5. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash by filling each well with Wash Buffer (350 uL) using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
    6. Add 100 uL of Detection Reagent B to each well. Cover with a new adhesive strip.Incubate for 1 hours at 37 °C.
    7. Repeat the aspiration/wash as in step
    5. 8. Add 90 uL of Substrate Solution to each well. Incubate for 30 minutes at room temperature. Protect from light.
    9. Add 50 uL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
    10. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
    Important Note:
    1. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
    2. It is recommended that no more than 32 wells be used for each assay run if manual pipetting is used since pipetting of all standards, specimens and controls should be completed within 5 minutes. A full plate of 96 wells may be used if automated pipetting is available.
    3. Duplication of all standards and specimens, although not required, is recommended.
    4. When mixing or reconstituting protein solutions, always avoid foaming. 4
    5. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
    6. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary.

    Calcul des résultats

    Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the Ferritin concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    1. The kit should not be used beyond the expiration date on the kit label.
    2. Do not mix or substitute reagents with those from other lots or sources.
    3. If samples generate values higher than the highest standard, further dilute the samples with the Assay Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
    4. This assay is designed to eliminate interference by soluble receptors, ligands, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Antigène Voir toutes FTH1 Kits ELISA
    FTH1 (Ferritin, Heavy Polypeptide 1 (FTH1))
    Autre désignation
    FTH1 (FTH1 Produits)
    Synonymes
    FHC Kit ELISA, FTH Kit ELISA, FTHL6 Kit ELISA, PIG15 Kit ELISA, PLIF Kit ELISA, apoferritin Kit ELISA, ferritin Kit ELISA, fhc Kit ELISA, fth Kit ELISA, fth1 Kit ELISA, fthl6 Kit ELISA, ftn-2 Kit ELISA, pig15 Kit ELISA, plif Kit ELISA, fth1b Kit ELISA, Fth Kit ELISA, HFt Kit ELISA, MFH Kit ELISA, fb06g09 Kit ELISA, hm:zeh1145 Kit ELISA, wu:fb06g09 Kit ELISA, wu:fq18c10 Kit ELISA, zeh1145 Kit ELISA, ferritin heavy chain 1 Kit ELISA, ferritin, heavy polypeptide 1 L homeolog Kit ELISA, ferritin, heavy polypeptide 1 S homeolog Kit ELISA, ferritin heavy polypeptide 1 Kit ELISA, ferritin, heavy polypeptide 1a Kit ELISA, ferritin Kit ELISA, ferritin, heavy polypeptide 1 Kit ELISA, tudor domain containing 9 Kit ELISA, ferritin, heavy polypeptide 1 a Kit ELISA, FTH1 Kit ELISA, fth1.L Kit ELISA, fth1.S Kit ELISA, Fth1 Kit ELISA, fth1a Kit ELISA, EAMY_RS19690 Kit ELISA, fth1 Kit ELISA, TDRD9 Kit ELISA
    Sujet
    Ferritin is an iron-protein complex formed from an intracellular acceptor called Apoferritin. Apoferritin is a large molecular weight 450,000 protein produced by the liver. Iron as Fe (HO)3 linked to apoferritin is then stored in the cytoplasm of the reticuloendothelial system, liver, spleen and bone marrow. Ferritin is the body's iron storage protein functioning primarily as a site for iron storage from which iron may be mobilized in response to such stimuli a dietary change, blood loss or pregnancy. The direct quantitation of serum ferritin offers the physician a convenient and accurate measure of total body iron stores, by means of diagnosing iron-deficiency and anemia due to such causes as inflammation and hepatic or renal disease. In addition, serum ferritin concentration may be useful in detecting iron overload, which may allow the detection of idiopathic hemachromatosis in the precirrhotic storage.
    Pathways
    Transition Metal Ion Homeostasis
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