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Fibronectin 1 Kit ELISA

FN1 Reactivité: Souris Colorimetric Sandwich ELISA 0.78-50 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN455123
  • Antigène Voir toutes Fibronectin 1 (FN1) Kits ELISA
    Fibronectin 1 (FN1)
    Reactivité
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.78-50 ng/mL
    Seuil minimal de détection
    0.78 ng/mL
    Application
    ELISA
    Fonction
    This immunoassay kit allows for the in vitro quantitative determination of mouse Fibronectin,FN concentrations in cell culture supernates, serum, plasma and other biological fluids.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This assay recognizes recombinant and natural mouse FN.
    Réactivité croisée (Details)
    No significant cross-reactivity or interference was observed.
    Sensibilité
    < 11.5 ng/mL
    The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.
    Attributs du produit
    Mus musculus,Mouse,Fibronectin,FN,Fn1
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    Discover our best selling FN1 Kit ELISA
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    The microtiter plate provided in this kit has been pre-coated with an antibody specific to FN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for FN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Stock
    4 °C/-20 °C
    Stockage commentaire
    The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.
  • Antigène Voir toutes Fibronectin 1 (FN1) Kits ELISA
    Fibronectin 1 (FN1)
    Autre désignation
    Fn1 (FN1 Produits)
    Synonymes
    FN1 Kit ELISA, FN Kit ELISA, cig Kit ELISA, fibronectin Kit ELISA, finc Kit ELISA, lets Kit ELISA, msf Kit ELISA, Fn Kit ELISA, fn2 Kit ELISA, fb80d10 Kit ELISA, wu:fb80d10 Kit ELISA, CIG Kit ELISA, ED-B Kit ELISA, FINC Kit ELISA, FNZ Kit ELISA, GFND Kit ELISA, GFND2 Kit ELISA, LETS Kit ELISA, MSF Kit ELISA, E330027I09 Kit ELISA, Fn-1 Kit ELISA, FIBNEC Kit ELISA, fn-1 Kit ELISA, fibronectin 1 Kit ELISA, fibronectin 1a Kit ELISA, fibronectin 1 S homeolog Kit ELISA, FN1 Kit ELISA, fn1 Kit ELISA, fn1a Kit ELISA, Fn1 Kit ELISA, fn1.S Kit ELISA
    Pathways
    Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy
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