FSHB Kit ELISA

N° du produit ABIN455139

Détail du produit FSHB Kit ELISA

Antigène: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.56-100 MioU/mL

Seuil minimal de détection: 1.56 MioU/mL

Application: ELISA

Fonction: This immunoassay kit allows for the specific measurement of Human Follicle-Stimulating Hormone (FSH) concentrations in serum and plasma.

Type d'échantillon: Serum, Plasma

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural Human FSH.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Homo sapiens,Human,Follitropin subunit beta,Follicle-stimulating hormone beta subunit,FSH-B,FSH-beta,Follitropin beta chain,FSHB

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for FSH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FSH present is bound by the immobilized antibody. An enzyme-linked polyclonal antibody specific for FSH is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color 2 develops in proportion to the amount of FSH bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Références pour FSHB Kit ELISA (ABIN455139)

Lv, Guo, Shi: "Effects of quinestrol on reproductive hormone expression, secretion, and receptor levels in female Mongolian gerbils (Meriones unguiculatus)." dans: Theriogenology, Vol. 77, Issue 6, pp. 1223-31, (2012) (PubMed).

Détail du antigène

Antigène: FSHB (Follicle Stimulating Hormone, beta Polypeptide (FSHB))

Autre désignation: FSHB (FSHB Produits)

Synonymes: gthI Kit ELISA, fsh-beta Kit ELISA, LOC100136594 Kit ELISA, FSHB Kit ELISA, LOC100218526 Kit ELISA, FSH Kit ELISA, Fshbeta Kit ELISA, FSH-beta Kit ELISA, follicle stimulating hormone, beta polypeptide Kit ELISA, follicle stimulating hormone beta subunit L homeolog Kit ELISA, follcle-stimulating hormone beta subunit Kit ELISA, follicle stimulating hormone beta subunit Kit ELISA, follicle stimulating hormone beta Kit ELISA, follitropin subunit beta Kit ELISA, fshb Kit ELISA, fshb.L Kit ELISA, LOC100136594 Kit ELISA, FSHB Kit ELISA, Fshb Kit ELISA

Sujet: Follicle stimulating hormone (FSH) is a hormone synthesised and secreted by gonadotropes in the anterior pituitary gland. In the ovary FSH stimulates the growth of immature Graafian follicles to maturation. FSH is a glycoprotein secreted by the basophil cells of the anterior pituitary. Gonadotropin-releasing hormone (GnRH), produced in the hypothalamus, controls the release of FSH from the anterior pituitary. Like other glycoproteins, such as LH, TSH, and HCG, FSH consists of subunits designated as alpha and beta. Hormones of this type have alpha subunits that are very similar in structure, therefore the biological and immunological properties of each hormone are dependent on the unique beta subunit. In the female, FSH stimulates the growth and maturation of ovarian follicles by acting directly on the receptors located on the granulosa cells, follicular steroidogenesis is promoted and LH production is stimulated. The LH produced then binds to the theca cells and stimulates steroidogenesis. Increased intraovarian estradiol production occurs as follicular maturation advances, thereupon stimulating increased FSH receptor activity and FSH follicular binding. FSH, LH, and estradiol are therefore intimately related in supporting ovarian recruitment and maturation in women.FSH levels are elevated after menopause, castration, and in premature ovarian failure. The levels of FSH may be normalized through the administration of estrogens, which demonstrate a negative feedback mechanism. Abnormal relationships between FSH and LH, and between FSH and estrogen have been linked to anorexia nervosa and polycystic ovarian disease. Although there are significant exceptions, ovarian failure is indicated when random FSH concentrations exceed 40 mIU/mL. The growth of the seminiferous tubules and maintenance of spermatogenesis in men are regulated by FSH. However, androgens, unlike estrogens, do not lower FSH levels, therefore demonstrating a feedback relationship only with serum LH.

Pathways: Peptide Hormone Metabolism, Hormone Activity, C21-Steroid Hormone Metabolic Process