HSPD1 Kit ELISA

N° du produit ABIN455304

Détail du produit HSPD1 Kit ELISA

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.156-10 ng/mL

Seuil minimal de détection: 0.156 ng/mL

Application: ELISA

Fonction: This immunoassay kit allows for the specific measurement of human heat shock protein 60 (HSP60) concentrations in cell culture supernates, serum, and plasma.

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human hsp60.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Attributs du produit: Homo sapiens,Human,60 kDa heat shock protein, mitochondrial,60 kDa chaperonin,Chaperonin 60,CPN60,Heat shock protein 60,HSP-60,Hsp60,HuCHA60,Mitochondrial matrix protein P1,P60 lymphocyte protein,

Ingrédients: Reagent (Quantity): Assay plate (1), 2 Standard (2), Sample Diluent (1x20ml), Assay Diluent A (1x10ml), Assay Diluent B (1x10ml), Detection Reagent A 1x120µl Detection Reagent B 1x120µl Wash Buffer(25 x concentrate) (1x30ml), Substrate (1x10ml), Stop Solution (1x10ml)

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for HSP60 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP60 present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for HSP60 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP60 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Préparation des réactifs:

Bring all reagents to room temperature before use. Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 500 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0 mL of Sample Diluent. This reconstitution produces a stock solution of 10,000 pg/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (10,000 pg/mL). The Sample Diluent serves as the zero standard (0 pg/mL). Detection Reagent A and B - Dilute to the working concentration specified on the vial label using 3 Assay Diluent A and B (1:100), respectively.

Prélèvement de l'échantillon: Cell culture supernates - Remove particulates by centrifugation and assay immediately or aliquot and store samples at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 x g. Remove serum and assay immediately or aliquot and store samples at -20 °C. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 x g at 2 - 8 °C within 30 minutes of collection. Store samples at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Note: Citrate plasma has not been validated for use in this assay.

Procédure de l'essai:

Allow all reagents to reach room temperature. Arrange and label required number of strips.
1. Prepare all reagents, working standards and samples as directed in the previous sections.
2. Add 100 uL of Standard, Control, or sample per well. Cover with the adhesive strip. Incubate for 2 hours at 37 °C.
3. Remove the liquid of each well, don’t wash.
4. Add 100 uL of Detection Reagent A to each well. Incubate for 1 hour at 37°C. Detection Reagent A may appear cloudy. Warm to room temperature and mix gently until solution appears uniform.
5. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash by filling each well with Wash Buffer (350 uL) using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
6. Add 100 uL of Detection Reagent B to each well. Cover with a new adhesive strip.Incubate for 1 hours at 37 °C.
7. Repeat the aspiration/wash as in step
5. 8. Add 90 uL of Substrate Solution to each well. Incubate for 30 minutes at room temperature. Protect from light.
9. Add 50 uL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
10. Determine the optical density of each well within 30 minutes, using a microplate reader set to 450 nm.
Important Note:
1. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
2. It is recommended that no more than 32 wells be used for each assay run if manual pipetting is used since pipetting of all standards, specimens and controls should be completed within 5 minutes. A full plate of 96 wells may be used if automated pipetting is available.
3. Duplication of all standards and specimens, although not required, is recommended.
4. When mixing or reconstituting protein solutions, always avoid foaming.
5. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
6. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. 4

Calcul des résultats:

Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the y-axis against the concentration on the x-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the HSP60 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: 1. The kit should not be used beyond the expiration date on the kit label.
2. Do not mix or substitute reagents with those from other lots or sources.
3. If samples generate values higher than the highest standard, further dilute the samples with the Assay Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique,incubation time or temperature, and kit age can cause variation in binding.
4. This assay is designed to eliminate interference by soluble receptors, ligands, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSPD1 (HSPD1 Produits)

Synonymes: CPN60 Kit ELISA, GROEL Kit ELISA, HLD4 Kit ELISA, HSP-60 Kit ELISA, HSP60 Kit ELISA, HSP65 Kit ELISA, HuCHA60 Kit ELISA, SPG13 Kit ELISA, chaperonin Kit ELISA, cpn60 Kit ELISA, groel Kit ELISA, hld4 Kit ELISA, hsp60 Kit ELISA, hsp65 Kit ELISA, spg13 Kit ELISA, 60kDa Kit ELISA, Hsp60 Kit ELISA, 12 Kit ELISA, BP5 Kit ELISA, CG12101 Kit ELISA, Cpn60 Kit ELISA, Dmel\\CG12101 Kit ELISA, Dmhsp60 Kit ELISA, G62 Kit ELISA, HSP60A Kit ELISA, Hsp60A Kit ELISA, IEF16 Kit ELISA, Mmp-P1 Kit ELISA, SSP 7506 Kit ELISA, d-hsp60 Kit ELISA, hsp60A Kit ELISA, l(1)10Ac Kit ELISA, l(1)BP5 Kit ELISA, l(1)G8 Kit ELISA, l(1)HM21 Kit ELISA, l(1)L12 Kit ELISA, l(1)dp025 Kit ELISA, cb863 Kit ELISA, fa04a05 Kit ELISA, fb22d10 Kit ELISA, fi27b05 Kit ELISA, id:ibd2197 Kit ELISA, sb:cb144 Kit ELISA, wu:fa04a05 Kit ELISA, wu:fb22d10 Kit ELISA, wu:fi04a12 Kit ELISA, wu:fi27b05 Kit ELISA, MIF4 Kit ELISA, MNA2 Kit ELISA, mopA Kit ELISA, groL Kit ELISA, crpA Kit ELISA, Hspd1-30p Kit ELISA, heat shock protein family D (Hsp60) member 1 Kit ELISA, heat shock protein family D (Hsp60) member 1 S homeolog Kit ELISA, 60 kDa heat shock protein, mitochondrial Kit ELISA, heat shock protein 1 (chaperonin) Kit ELISA, Heat shock protein 60A Kit ELISA, heat shock 60 protein 1 Kit ELISA, chaperone ATPase HSP60 Kit ELISA, molecular chaperone GroEL Kit ELISA, thermosome subunit Kit ELISA, chaperonin GroEL Kit ELISA, mitochondrial chaperonin Kit ELISA, heat shock protein family D member 1 Kit ELISA, HSPD1 Kit ELISA, hspd1.S Kit ELISA, hspd1 Kit ELISA, LOC100414401 Kit ELISA, Hspd1 Kit ELISA, Hsp60A Kit ELISA, HSP60 Kit ELISA, groEL Kit ELISA, MMP_RS07785 Kit ELISA, groEl Kit ELISA, LOC100136430 Kit ELISA

Sujet: Heat shock protein 60s (hsp60) are remarkably immunogenic, and both T-cell and antibody responses to hsp60 have been reported in various inflammatory conditions. Analysis of the nucleotide sequences of the TCR demonstrated that human hsp60-reactive T-cell clones and periodontitis lesion-infiltrating T cells have the same receptors, suggesting that hsp60-reactive T cells accumulate in periodontitis lesions. Analysis of the cytokine profile demonstrated that hsp60-reactive PBMC produced significant levels of gamma interferon (IFN-gamma) in periodontitis patients, whereas P. gingivalis GroEL did not induce any skewing toward a type1 or type2 cytokine profile. In control subjects no significant expression of IFN-gamma or interleukin 4 was induced. These results suggest that periodontitis patients have human hsp60-reactive T cells with a type 1 cytokine profile in their peripheral blood T-cell pools. Heat shock protein 60 (HSP60) is homologous to HSP65 (a major antigenic mycobacterial protein) and E. coli GroEL. HSP60, also referred to as chaperonin-60, P1 and mitonin, has been classified as a member of a family of proteins termed chaperonins which act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly. HSP60 is an abundant protein which is constitutively expressed and is induced by environmental stress. HSP60 exists as a large oligomer composed of 14 ~60 kDa subunits arranged as two stacked rings.

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity