AGLU Kit ELISA

N° du produit ABIN455810

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain AGLU.

Aperçu rapide pour AGLU Kit ELISA (ABIN455810)

Antigène: AGLU (alpha-Glucosidase (AGLU))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Type d'échantillon: Tissue Homogenate

Détail du produit AGLU Kit ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of human MAL concentrations in tissue homogenates and other biological fluids.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural human MAL.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 0.78 U/L
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Homo sapiens,Human,Maltase-glucoamylase, intestinal,MGAM,MGA,MGAML

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MAL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MAL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: AGLU (alpha-Glucosidase (AGLU))

Autre désignation: MGAM

Sujet: Maltase (acid maltase, alpha-1,4-glucosidase) is an enzyme produced by the cells lining the small intestine that breaks down the disaccharide maltose. It comes under the enzyme category carbohydrase (which is a subcategory of hydrolase). Maltase is secreted by the surface cells of the villi, which are thin projections on the mucosa. These are found throughout the small intestine, but differ in shape in the duodenum and ileum sections. It must also be located in other places, like the heart, if a deficiency of it causes Pompe's Disease (which results in cardiomegaly).

Pathways: Cellular Glucan Metabolic Process