Osteocalcin Kit ELISA

N° du produit ABIN456053

Détail du produit Osteocalcin Kit ELISA

Antigène: Osteocalcin (BGLAP)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: This immunoassay kit allows for the in vitro quantitative determination of mouse Osteocalcin/Bone gla protein, OT/BG concentrations in serum, plasma and other biological fluids.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse OT.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Sensibilité: < 3.9 pg/mL
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest detectable concentration that could be differentiated from zero.

Attributs du produit: Mus musculus,Mouse,Osteocalcin,Bone Gla protein,BGP,Gamma-carboxyglutamic acid-containing protein,Bglap

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: The microtiter plate provided in this kit has been pre-coated with an antibody specific to OT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for OT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain OT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 2 nm. The concentration of OT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Détail du antigène

Antigène: Osteocalcin (BGLAP)

Autre désignation: Bglap (BGLAP Produits)

Synonymes: BGP Kit ELISA, OC Kit ELISA, OCN Kit ELISA, Bglap1 Kit ELISA, Bglap2 Kit ELISA, OG1 Kit ELISA, mOC-A Kit ELISA, Bgp Kit ELISA, Bgpr Kit ELISA, Bgpra Kit ELISA, bgp Kit ELISA, LOC792433 Kit ELISA, ocn Kit ELISA, GLA Kit ELISA, BGLAP Kit ELISA, PMF1 Kit ELISA, bone gamma-carboxyglutamate protein Kit ELISA, bone gamma carboxyglutamate protein Kit ELISA, bone gamma-carboxyglutamate protein S homeolog Kit ELISA, bone gamma-carboxyglutamate (gla) protein Kit ELISA, osteocalcin Kit ELISA, polyamine-modulated factor 1 Kit ELISA, BGLAP Kit ELISA, Bglap Kit ELISA, bglap.S Kit ELISA, bglap Kit ELISA, ocn Kit ELISA, LOC100529177 Kit ELISA, LOC100146589 Kit ELISA

Sujet: Osteocalcin (OT) is the most abundant noncollagenous protein in mature bone where it constitutes 1% to 2% of the total protein. Synthesized by osteoblasts, it is incorporated into the bone matrix. Osteocalcin is a 49-residue protein with three gamma-carboxyglutamic acid residues, at positions 17, 21 and 24. These three residues confer on it a very strong ability to bind to hydroxyapatite. Osteocalcin is a noncollagenous protein found in bone and dentin. It is secreted by osteoblasts and thought to play a role in mineralization and calcium ion homeostasis. It has been stipulated that osteocalcin may also function as a negative regulator of bone formation, although its exact role is unknown. It was reported that osteocalcin acts as a hormone in the body, causing beta cells in the pancreas to release more insulin, and at the same time directing fat cells to release the hormone adiponectin, which increases sensitivity to insulin. As osteocalcin is manufactured by osteoblasts, it is often used as a biochemical marker, or biomarker, for the bone formation process. It has been routinely observed that higher serum-osteocalcin levels are relatively well correlated with increases in bone mineral density (BMD) during treatment with anabolic bone formation drugs for osteoporosis, such as Forteo. In many studies, Osteocalcin is used as a preliminary biomarker on the effectiveness of a given drug on bone formation.