Osteopontin Kit ELISA

N° du produit ABIN456061

Kit ELISA Osteopontin Souris, Colorimetric test pour la quantification de Souris Osteopontin et a été mentionné dans 1 publication.

Aperçu rapide pour Osteopontin Kit ELISA (ABIN456061)

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.312-20 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma

Détail du produit Osteopontin Kit ELISA

Seuil minimal de détection: 0.312 ng/mL

Fonction: This immunoassay kit allows for the specific measurement of Mouse Osteopontin (OPN) concentrations in cell culture supernates, serum and plasma.

Analytical Method: Quantitative

Specificité: This assay recognizes recombinant and natural mouse Osteopontin.

Réactivité croisée (Details): No significant cross-reactivity or interference was observed.

Attributs du produit: Mus musculus,Mouse,Osteopontin,2AR,Bone sialoprotein 1,Calcium oxalate crystal growth inhibitor protein,Early T-lymphocyte activation 1 protein,Minopontin,Secreted phosphoprotein 1,SPP-1,Spp1,Eta-1,Op,Spp-1

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for Osteopontin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Osteopontin present is bound by the immobilized antibody. An enzyme-linked monoclonal antibody specific for Osteopontin is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound in the initial step. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Stockage commentaire: The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20 °C upon being received. The other reagents can be stored at 4 °C.

Références pour Osteopontin Kit ELISA (ABIN456061)

Yang, Cao, Wang, Tong, Shi, Wu, Zheng, Guo, Pan, Gao, Wang: "Proliferation and osteogenesis of immortalized bone marrow-derived mesenchymal stem cells in porous polylactic glycolic acid scaffolds under perfusion culture." dans: Journal of biomedical materials research. Part A, Vol. 92, Issue 3, pp. 817-29, (2010) (PubMed).

Détail du antigène

Antigène: Osteopontin (SPP1) (Secreted phosphoprotein 1 (SPP1))

Autre désignation: Spp1

Sujet: Osteopontin (OPN), also known as early T lymphocyte activation 1 (Eta-1), is a secreted multifunctional glyco-phosphoprotein with roles in bone metabolism, immune regulation, tissue remodeling , cell survival, and tumor progression . Gene structure and chromosomal location identify OPN as a member of the small integrin-binding ligand N-linked glycoprotein (SIBLING) family that also includes bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin sialophosphoprotein(DSPP), enamelin (ENAM), and matrix extracellular phosphoglycoprotein (MEPE). Murine OPN issynthesized as a 294 amino acid (aa) precursor protein with a predicted 16 aa signal peptide and a highly unusual mature protein sequence, containing 68 acidic aa and 23 potential Ser/Thr phosphorylation sites. Although the predicted molecular weight of OPN is 31 kDa,phosphorylation and N- and O-glycosylation may allow it to appear as large as 75 kDa. Variability in post-translational modifications can influence the activity of OPN. Osteopontin, as indicated by its name meaning “bone bridging”, is highly expressed in mineralized tissues. It is also expressed in other tissues including cartilage, kidney, vascular tissues, activated macrophages, lymphocytes, and epithelia. A portion of cell expressed OPN is also retained intracellularly. In vitro, OPN stimulates the adhesion of osteoclasts to bone, and bone resorption is blocked by inhibition of this interaction. Knockout mice have outwardly normal bone development, but exhibit deficient postnatal bone resorption in several contexts, supporting a role for OPN in osteoclast function. In kidney epithelia, OPN is upregulated by high concentrations of oxalate and inhibits calcium oxalate crystal nucleation and growth. In endothelial and smooth muscle cells, OPN is upregulated by high phosphate concentration and during atherosclerosis, binding of OPN to hydroxyapatite inhibits calcification of blood vessels and heart valves. OPN expression by macrophages and T cells is upregulated by inflammatory mediators including LPS,NO, IL-1β, and TNF-α. OPN regulates macrophage differentiation and recruitment. It also functions as a chemotactic factor and co-stimulator of T cells and may act as a Th1 cytokine, stimulating IL-12 production. OPN production by macrophages is upregulated at sites of tissue remodeling including the placenta, endometrium and myocardium post-infarction. OPN is expressed by many tumor types and plays a role in tumor progression and metastasis. OPN may also be upregulated during autoimmune processes such as rheumatoid arthritis or in 2 granulomatous lesions in response to infections such as tuberculosis.

Pathways: Regulation of Cell Size