IgM Kit ELISA
Aperçu rapide pour IgM Kit ELISA (ABIN457299)
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Méthode de détection
Type de méthode
Application
Type d'échantillon
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Seuil minimal de détection
- 156 ng/mL
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Fonction
- The IgM(pig)-ELISA is designed for the quantitative determination of pig IgM in complex samples (serum or other biological samples).
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Analytical Method
- Quantitative
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Attributs du produit
- The determination of pig IgM is carried out as direct sandwich ELISA.
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Plaque
- Pre-coated
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Protocole
- An antibody specific for pig IgM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgM present is bound. After washing away any unbound substances, an enzyme-linked antibody is added. Following a wash, a substrate solution is added to the wells and color develops in proportion to the amount of antibody conjugate. The absorption at 450 nm is proportional to the IgM concentration.
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Précision du teste
- Intraassay precision (CV): (n=10) at 57 ng/ml: 5.3% at 218 ng/ml: 2.7% at 580 ng/ml: 4.7%
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- IgM
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Classe de substances
- Antibody
Antigène Voir toutes IgM Kits ELISA
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