Anti-Complement Factor H Antibody Kit ELISA

N° du produit ABIN459353

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain .

Aperçu rapide pour Anti-Complement Factor H Antibody Kit ELISA (ABIN459353)

Antigène: Anti-Complement Factor H Antibody

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum, Plasma

Détail du produit

Fonction: anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H.

Marque: ELISA-VIDITEST

Analytical Method: Quantitative

Ingrédients:

• ELISA strips coated with human factor H purified from human plasma STRIPS Ag 1 x 6 strips
• 50 μL Anti-CFH IgG standard STANDARD 1 vial
• 0.1 mL IgG-HRP conjugate - detection anti-human antibody labelled by horseradish peroxidase,101x concentrated CONJ 101x 1 vial
• 55 mL Wash buffer 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer (DB) r.t.u. 1) DIL 1 vial
• 13 mL Chromogenic substrate (TMB-BF substrate) r.t.u. (TMB/H2O2) TMB-BF 1 vial
• 13 mL Stop solution r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality control certificate 1) r.t.u. ready to use

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-complement factor H is an enzyme linked immunosorbent assay designed to detect IgG antibodies against complement factor H. The wells of the microtitrate plate are coated with purified human complement factor H. Antibodies against factor H present in serum sample bind to the immobilized factor H. Other antibodies, unbound to the factor H, are washed away during the next step. Then anti-human IgG antibodies labelled with horseradish peroxidase are added and those detect the antibodies from the sample that previously bound to factor H. The unbound labelled antibodies are washed away and the remaining labelled antibodies are visualized with a chromogenic substrate. The peroxidase activity leads to a change in colour of the solution. The reaction is stopped by adding an acidic solution. The colour intensity is directly proportional to the amount of anti-factor H antibodies in the sample.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Anti-Complement Factor H Antibody

Classe de substances: Antibody