EBV EA(D) IgG Kit ELISA

N° du produit ABIN459354

Ce kit ELISA Humain EBV EA(D) IgG est un kit ELISA Colorimetric conçu pour quantifier Humain EBV EA(D) IgG.

Aperçu rapide pour EBV EA(D) IgG Kit ELISA (ABIN459354)

Antigène: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit EBV EA(D) IgG Kit ELISA

Fonction: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 1.3 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-EA (D)EBV IgG assay is a solid-phase immunoanalytical test. The polystyrene strips are coated with a specific recombinant antigen containing immunodominant epitopes of EA antigen. Anti-EA (D) antibodies from samples bind to the immobilized antigens. The other serum antibodies do not bind and are washed away those that formed complexes with the antigens are later on recognized by animal anti-human IgG antibodies labelled with horseradish peroxidase. The presence of the labelled antibodies is revealed by an enzymatic reaction with a chromogenic substrate. Negative samples do not react and the mild change in colour, if present, may be attributed to the reaction background.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: EBV EA(D) IgG (Anti-Epstein-Barr Virus Early Antigen (D) IgG (EBV EA(D) IgG))

Autre désignation: Anti-EA (D) Epstein-Barr Virus (EBV) IgG Antibody

Classe de substances: Antibody