EBV NA IgG Kit ELISA
Aperçu rapide pour EBV NA IgG Kit ELISA (ABIN459361)
Antigène
Tous les produits EBV NA IgGReactivité
Méthode de détection
Type de méthode
Application
Type d'échantillon
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Fonction
- anti-EBNA-1 EBV IgG is a solid-phase immunoanalytical test.
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Marque
- ELISA-VIDITEST
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Analytical Method
- Semi-Quantitative
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Ingrédients
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- ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
- 1.3 mL Standard A = Negative control human serum, r.t.u. 1) ST A/NC 1 vial
- 1.3 mL Standard B (human serum) r.t.u. ST B 1 vial
- 1.3 mL Standard C (human serum) r.t.u. ST C 1 vial
- 2.0 mL Standard D = Calibrator (human serum), r.t.u. ST D/CAL 1 vial
- 1.3 mL Standard E = Positive control human serum, r.t.u. ST E/PC 1 vial
- 13 mL Anti-human IgG animal antibodies labelled with horseradish peroxidase (anti-IgG Px conjugate) r.t.u. CONJ 1 vial
- 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
- 60 mL Dilution buffer, r.t.u. DIL 1 vial
- 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O 2) TMB 1 vial
- 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
- Instruction manual
- Quality Control Certificate
- 1) r.t.u., ready to use
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Matériel non inclus
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- Distilled or deionised water for dilution of buffer and standard concentrates
- appropriate and calibrated equipment for pipetting
- temperature controlled incubator
- spectrophotometer or platereader with the appropriate filters
- empty microtiter plate for pre-incubation
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Plaque
- Pre-coated
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Protocole
- anti-EBNA-1 EBV IgG is a solid-phase immunoanalytical test. A specific recombinant antigen of EBNA-1 EBV is bound to the surface of the wells. If relevant antibodies are present in the test samples, they bind to the immobilized antigens. In the next step, the bound antibodies then react with horseradish peroxidase-labeled anti-human IgG antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.
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Restrictions
- For Research Use only
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Stock
- 4 °C
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- EBV NA IgG (Anti-Epstein-Barr Virus Nuclear Antigen IgG (EBV NA IgG))
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Classe de substances
- Antibody
Antigène Tous les produits EBV NA IgG
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