EBV VCA IgA Kit ELISA

N° du produit ABIN459374

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain EBV VCA IgA.

Aperçu rapide pour EBV VCA IgA Kit ELISA (ABIN459374)

Antigène: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit EBV VCA IgA Kit ELISA

Fonction: anti-VCA EBV IgA is a solid-phase immunoanalytical test.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgA animal antibodies labelled with horseradish peroxidase (anti-IgA Px conjugate) r.t.u. CONJ 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H 2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-VCA EBV IgA is a solid-phase immunoanalytical test. A specific antigen representing immunodominant epitopes of the VCA complex is bound to the surface of the wells. If antibodies are present in the test samples, they will bind to the immobilized proteins. The bound antibodies then react in the next step with horseradish peroxidase-labeled anti-human IgA antibodies. The amount of bound labeled antibodies is determined by a color enzymatic reaction. Negative samples do not react, a slight change in the color of the wells is the background of the reaction.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: EBV VCA IgA (Anti-Epstein-Barr Virus Viral Capsid Antigen IgA (EBV VCA IgA))

Classe de substances: Antibody