EBV VCA IgM Kit ELISA

N° du produit ABIN459379

Kit ELISA EBV VCA IgM Humain, Colorimetric test pour la quantification de Humain EBV VCA IgM.

Aperçu rapide pour EBV VCA IgM Kit ELISA (ABIN459379)

Antigène: EBV VCA IgM (Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Indirect ELISA

Application: ELISA

Type d'échantillon: Serum

Détail du produit EBV VCA IgM Kit ELISA

Fonction: ELISA-VIDITEST anti-VCA EBV IgM assay is a solid-phase immunoanalytical test.

Marque: ELISA-VIDITEST

Analytical Method: Semi-Quantitative

Ingrédients:

• ELISA break-away strips in the handling frame coated with the specific antigen STRIPS Ag 1 x 12 pcs
• 1.3 mL Negative control human serum, r.t.u. 1) NC 1 vial
• 1.3 mL Positive control human serum, r.t.u. PC 1 vial
• 2.0 mL Calibrator (human serum), r.t.u. CAL 1 vial
• 13 mL Anti-human IgM animal antibodies labelled with horseradish peroxidase (anti-IgM Px conjugate) r.t.u. CONJ-V 1 vial
• 55 mL Wash buffer, 10x concentrated WASH 10x 1 vial
• 60 mL Dilution buffer, r.t.u. DIL 1 vial
• 13 mL Chromogenic substrate TMB, r.t.u. (TMB/H2O2) TMB 1 vial
• 13 mL Stop solution, r.t.u. (0.4 M sulfuric acid) STOP 1 vial
• 2 mL RF-sorbent (anti-human IgG) 25 x concentrated RF SORB 25x 1 vial
• Instruction manual
• Quality Control Certificate
• 1) r.t.u., ready to use

Matériel non inclus:

• Distilled or deionised water for dilution of buffer and standard concentrates
• appropriate and calibrated equipment for pipetting
• temperature controlled incubator
• spectrophotometer or platereader with the appropriate filters
• empty microtiter plate for pre-incubation

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: anti-VCA EBV IgM assay is a solid-phase immunoanalytical test. The strips are coated with specific antigen that bears immunodominant epitopes of VCA complex. If relevant antibodies are present in the test samples, they bind to the immobilized antigen. The bound antibodies then in a next step react with horseradish peroxidase-labeled anti-human IgM antibodies. The amount of labeled antibodies bound is determined by the color enzymatic reaction. Negative samples do not react, a mild change in the colour of the wells is the reaction background.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: EBV VCA IgM (Anti-Epstein-Barr Virus Viral Capsid Antigen IgM (EBV VCA IgM))

Classe de substances: Antibody