Amylin/DAP Kit ELISA

N° du produit ABIN4881297

Kit ELISA Humain, Souris et Rat Amylin/DAP, test Colorimetric pour la quantification de Humain, Souris et Rat Amylin/DAP.

Aperçu rapide pour Amylin/DAP Kit ELISA (ABIN4881297)

Antigène: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Reactivité: Humain, Souris, Rat

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum

Détail du produit Amylin/DAP Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Amylin (IAPP) EIA Kit optimized for serum and cell culture medium. Competition-based ELISA on a 96-well strip plate.

Analytical Method: Quantitative

Specificité: Cross Reactivity: This EIA kit shows no cross-reactivity with any of the cytokines tested: Ghrelin, Nesfatin, Angiotensin II, NPY and APC.

Sensibilité: 0.62 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated Amylin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Amylin/DAP (IAPP) (Islet Amyloid Polypeptide (IAPP))

Autre désignation: Amylin

ID gène: 3375

UniProt: P10997

Pathways: Hormone Activity, Feeding Behaviour