CAMP Kit ELISA

N° du produit ABIN4881300

Détail du produit CAMP Kit ELISA

Antigène: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Reactivité: Chemical

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1-1.000 ng/mL

Seuil minimal de détection: 0.1 ng/mL

Application: ELISA

Fonction: cAMP EIA Kit optimized for serum and plasma samples. Competition-based ELISA on a 96-well strip plate.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This EIA kit is designed to only detect Cyclic AMP.

Sensibilité: 4.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated cAMP peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Autre désignation: cAMP, Cyclic AMP (cAMP Produits)

Synonymes: CRAMP Kit ELISA, CAP-18 Kit ELISA, CAP18 Kit ELISA, FALL-39 Kit ELISA, FALL39 Kit ELISA, LL37 Kit ELISA, CAP11 Kit ELISA, Cnlp Kit ELISA, Cramp Kit ELISA, MCLP Kit ELISA, CATHL7 Kit ELISA, cathelicidin antimicrobial peptide Kit ELISA, Camp Kit ELISA, CAMP Kit ELISA

Classe de substances: Chemical

Pathways: Cellular Response to Molecule of Bacterial Origin