CAMP Kit ELISA

N° du produit ABIN4881300

Kit ELISA CAMP Humain, Colorimetric test pour la quantification de Humain CAMP.

Aperçu rapide pour CAMP Kit ELISA (ABIN4881300)

Antigène: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit CAMP Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: cAMP EIA Kit optimized for serum and plasma samples. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This EIA kit is designed to only detect Cyclic AMP.

Sensibilité: 4.4 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: CAMP (cAMP) (Cyclic Adenosine Monophosphate (cAMP))

Autre désignation: cAMP, Cyclic AMP

Classe de substances: Chemical

Sujet: Optional[Gene Symbols]: Cyclic adenosine monophosphate (cAMP, cyclic AMP, or 3",5"-cyclic adenosine monophosphate) is a second messenger important in many biological processes. cAMP is derived from adenosine triphosphate (ATP) and used for intracellular signal transduction in many different organisms, such as transferring into cells the effects of hormones like glucagon and adrenaline, which cannot pass through the plasma membrane. cAMP functions through three main effectors: PKA, the guanine-nucleotide-exchange factor (GEF) EPAC and cyclic-nucleotide-gated ion channels. The intracellular levels of cAMP are regulated by the balance between the activities of two enzymes adenylyl cyclase (AC) and cyclic nucleotide phosphodiesterase (PDE).

Cyclic AMP has been shown to be involved in cell growth, differentiation and general metabolism, and it is important for many biological function, especially in the cardiovascular, nervous and immune systems. The measurement of intracellular Cyclic AMP in tissues and cell cultures may help to provide a clearer understanding of the physiology and pathology of many disease states.

Pathways: Cellular Response to Molecule of Bacterial Origin