NUCB2 Kit ELISA

N° du produit ABIN4881312

Kit ELISA NUCB2 Humain, Rat et Souris, Colorimetric test pour la quantification de Humain, Rat et Souris NUCB2 et a été mentionné dans 2+ publications.

Aperçu rapide pour NUCB2 Kit ELISA (ABIN4881312)

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit NUCB2 Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Nesfatin EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This kit detects human, mouse and rat 82aa Nesfatin protein. No other active isoforms have been reported.

Sensibilité: 400 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

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• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Suggested Plasma Dilution: Human: 2X / Mouse: 2X / Rat: 2X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Références pour NUCB2 Kit ELISA (ABIN4881312)

Serin, Bakacak, Ercan, Köstü, Avci, Arıkan, Kıran: "The evaluation of Nesfatin-1 levels in patients with and without intrauterine growth restriction." dans: The journal of maternal-fetal & neonatal medicine, Vol. 29, Issue 9, pp. 1409-13, (2016) (PubMed).

Abaci, Catli, Anik, Kume, Bober: "The relation of serum nesfatin-1 level with metabolic and clinical parameters in obese and healthy children." dans: Pediatric diabetes, Vol. 14, Issue 3, pp. 189-95, (2013) (PubMed).

Détail du antigène

Antigène: NUCB2 (NES1) (Nesfatin-1 (NES1))

Autre désignation: Nesfatin

Sujet:

Gene Symbols: NUCB2, NEFA

Background: Obesity, which is characterized by excessive accumulation of adipose tissue in the body, has become one of the greatest public health challenges. Obesity is not only associated with health problems linked to increased weight-dependent pressure overload on lung, joints and bones, but also a important risk factor for life-threatening diseases such as cardiovascular diseases, type 2 diabetes and certain cancers.

Nesfatin is a naturally occurring hormone produced by brain cells. Japanese scientists discovered in 2006 that it is responsible for regulating appetite and producing body fat. Excess Nesfatin in the brain leads to a loss of appetite, less frequent hunger, a "sense of fullness", and a drop in body fat and weight. A lack of Nesfatin in the brain leads to an increase of appetite, more frequent episodes of hunger, an increase of body fat and weight, and the inability to "feel full." This latter condition can be artificially induced by injecting an anti-Nesfatin antibody into the brain.

The receptors of Nesfatin within the brain are not completely understood, although they are thought to be contained in the hypothalamus and in the solitary nucleus, where Nesfatin is believed to produced via peroxisome proliferator-activated receptors (PPARs).

Protein Name (Synonyms): Nucleobindin-2 (DNA-binding protein NEFA) (Gastric cancer antigen Zg4) (Prepronesfatin) [Cleaved into: Nesfatin-1]

ID gène: 4925

UniProt: P80303