Neurotensin Kit ELISA

N° du produit ABIN4881315

Kit ELISA Neurotensin Humain, Rat et Souris, Colorimetric test pour la quantification de Humain, Rat et Souris Neurotensin.

Aperçu rapide pour Neurotensin Kit ELISA (ABIN4881315)

Antigène: Neurotensin (NTS)

Reactivité: Humain, Rat, Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 0.1 ng/mL - 1000 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Serum

Détail du produit Neurotensin Kit ELISA

Seuil minimal de détection: 0.1 ng/mL

Fonction: Human/Mouse/Rat Neurotensin EIA Kit optimized for serum, plasma and cell culture supernatants. Competition-based ELISA

Analytical Method: Quantitative

Specificité: This EIA kit is designed to detect human, mouse, and rat Neurotensin .

Sensibilité: 0.2 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Standard Peptide
• Assay Diluent(s)
• Biotinylated Peptide
• HRP-Streptavidin
• TMB One-Step Substrate
• Stop Solution
• Assay Diagram
• Positive Control Sample
• Capture Antibody
• User Manual

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Orbital shaker
• Aluminum foil
• Saran Wrap
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• SigmaPlot software (or other software that can perform four-parameter logistic regression models)

Information d'application

Indications d'application: Suggested Plasma Dilution: Human: 2X / Mouse: 8X / Rat: 8X

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed.
2. Add 100 μL detection antibody to each well.
3. Incubate 1.5 h at RT or O/N at 4 °C.
4. Add 100 μL standard or sample to each well.
5. Incubate 2.5 h at RT.
6. Add 100 μL prepared streptavidin solution.
7. Incubate 45 min at RT.
8. Add 100 μL TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL Stop Solution to each well.
11. Read plate at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Standard, Biotinylated Neurotensin peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.

Date de péremption: 6 months

Détail du antigène

Antigène: Neurotensin (NTS)

Autre désignation: Neurotensin

Sujet:

Gene Symbols: NTS

Background: Neurotensin is a 13 amino acid neuropeptide that is widely distributed throughoutthe nervous system, and may function as a neurotransmitter or a neuromodulator. Itacts on three subtypes of neurotensin receptors which have many central andperipheral actions.

Neurotensin induces a variety of effects, including: analgesia, hypothermia andincreased locomotor activity. It may be involved in dopamine-associatedpathophysiological events.

In the periphery, Neurotensin is found in enteroendocrine cells of the smallintestine. It may be involved in the maintenance of gut structure and function, and inthe regulation of fat metabolism. It is a potent mitogen for colorectal cancer.Neurotensin also exhibits antimicrobial activity against bacteria and fungi.

Protein Name (Synonyms): Neurotensin/neuromedin N [Cleaved into: Large neuromedin N (NmN-125) Neuromedin N (NN) (NmN) Neurotensin (NT) Tail peptide]

ID gène: 4922

UniProt: P30990