FIL1d Kit ELISA

N° du produit ABIN4881461

Détail du produit FIL1d Kit ELISA

Antigène: FIL1d (Interleukin 1 delta (FIL1d))

Reactivité: Boeuf (Vache)

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Bovine IL-1 F5 (IL36RN/FIL1 delta) ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: The antibody pair provided in this kit recognizes Bovine IL-1 F5 (IL36RN/FIL1 delta)

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FIL1d (Interleukin 1 delta (FIL1d))

Autre désignation: IL-36 Ra (FIL1d Produits)

Synonymes: AI413231 Kit ELISA, Fil1delta Kit ELISA, IL36RN Kit ELISA, Il-1h3 Kit ELISA, Il1hy1 Kit ELISA, FIL1 Kit ELISA, FIL1(DELTA) Kit ELISA, FIL1D Kit ELISA, IL1F5 Kit ELISA, IL1HY1 Kit ELISA, IL1L1 Kit ELISA, IL1RP3 Kit ELISA, IL36RA Kit ELISA, PSORP Kit ELISA, Il1f5 Kit ELISA, interleukin 1 family, member 5 (delta) Kit ELISA, interleukin 36 receptor antagonist Kit ELISA, Il1f5 Kit ELISA, IL36RN Kit ELISA, Il36rn Kit ELISA