Angiotensin I Converting Enzyme 1 Kit ELISA
-
- Antigène Voir toutes Angiotensin I Converting Enzyme 1 (ACE) Kits ELISA
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
-
Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Custom Human ACE (CD143) ELISA Kit.
- Type d'échantillon
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- Specificité
- This ELISA antibody pair recognizes human ACE. Other species not yet determined.
- Attributs du produit
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling ACE Kit ELISA
- Top Product
- Discover our top product ACE Kit ELISA
-
-
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
-
- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
-
- Antigène Voir toutes Angiotensin I Converting Enzyme 1 (ACE) Kits ELISA
- Angiotensin I Converting Enzyme 1 (ACE) (Angiotensin I Converting Enzyme (Peptidyl-Dipeptidase A) 1 (ACE))
- Autre désignation
- ACE (ACE Produits)
- Synonymes
- ACE1 Kit ELISA, CD143 Kit ELISA, DCP Kit ELISA, DCP1 Kit ELISA, ICH Kit ELISA, MVCD3 Kit ELISA, AW208573 Kit ELISA, Dcp1 Kit ELISA, StsRR92 Kit ELISA, dcp Kit ELISA, ace1 Kit ELISA, dcp1 Kit ELISA, xace Kit ELISA, cd143 Kit ELISA, ACE Kit ELISA, angiotensin I converting enzyme Kit ELISA, angiotensin I converting enzyme (peptidyl-dipeptidase A) 1 Kit ELISA, angiotensin-converting enzyme Kit ELISA, angiotensin I converting enzyme (peptidyl-dipeptidase A) 3 Kit ELISA, angiotensin-converting enzyme-like Kit ELISA, ACE Kit ELISA, Ace Kit ELISA, ace Kit ELISA, CpipJ_CPIJ009106 Kit ELISA, ACE3 Kit ELISA, LOC101824864 Kit ELISA
- ID gène
- 1636
- UniProt
- P12821
- Pathways
- ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Feeding Behaviour, Smooth Muscle Cell Migration
-