AHCY Kit ELISA

N° du produit ABIN4881774

Kit ELISA AHCY Humain, Colorimetric test pour la quantification de Humain AHCY.

Aperçu rapide pour AHCY Kit ELISA (ABIN4881774)

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.61 ng/mL - 150 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit AHCY Kit ELISA

Seuil minimal de détection: 0.61 ng/mL

Fonction: Human AHCY ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin C, APE, Bax, BNIP3, Caspase-3, Cathepsin F, CD177, CD96, CLEC4D, C-Peptide, DCBLD2, Dystroglycan, FAM3A, GCNT1, GLA, HAPLN4, hCG alpha, HMGB3, IkB-E, IRF4, KIR3DL1, Klotho beta, Lck, LILRA5, METTL11A, p55PIK, PCDH17, PLA2G4A, RalB, S100A2, S100B, SH2B1, Siglec-3, SOD3, ST8SIA2, Testican 1, TRP14, VAP-B, ZAP70.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: AHCY (Adenosylhomocysteinase (AHCY))

Autre désignation: AHCY

Sujet:

Gene Symbols: AHCY, SAHH

Protein Name (Synonyms): Adenosylhomocysteinase (AdoHcyase) (EC 3.3.1.1) (S-adenosyl-L-homocysteine hydrolase)

ID gène: 191

UniProt: P23526