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INA Kit ELISA
INA
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4881780
Détail du produit INA Kit ELISA
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Antigène
Voir toutes INA Kits ELISA
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Reactivité
Toutes les réactivités sur INA Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Application
ELISA
Fonction
Custom Human Alpha-Internexin ELISA Kit.
Type d'échantillon
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificité
This ELISA antibody pair recognizes Human C3a.
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes INA Kits ELISA
INA
(Internexin Neuronal Intermediate Filament Protein, alpha (INA))
Autre désignation
alpha-Internexin (INA Produits )
Synonymes
NEF5 Kit ELISA, NF-66 Kit ELISA, TXBP-1 Kit ELISA, Inexa Kit ELISA, Intlaa Kit ELISA, Nf66 Kit ELISA, AV028420 Kit ELISA, NF66 Kit ELISA, ina-A Kit ELISA, xefiltin Kit ELISA, ina-a Kit ELISA, inexa Kit ELISA, MGC82496 Kit ELISA, INA Kit ELISA, si:ch211-67n3.6 Kit ELISA, nef5 Kit ELISA, xina Kit ELISA, nf-66 Kit ELISA, txbp-1 Kit ELISA, MGC69277 Kit ELISA, gefiltin Kit ELISA, zgc:110516 Kit ELISA, internexin neuronal intermediate filament protein alpha Kit ELISA, internexin neuronal intermediate filament protein, alpha Kit ELISA, internexin neuronal intermediate filament protein alpha S homeolog Kit ELISA, low molecular weight neuronal intermediate filament S homeolog Kit ELISA, internexin neuronal intermediate filament protein, alpha a Kit ELISA, internexin neuronal intermediate filament protein, alpha b Kit ELISA, INA Kit ELISA, Ina Kit ELISA, ina.S Kit ELISA, nif.S Kit ELISA, inaa Kit ELISA, ina Kit ELISA, inab Kit ELISA
ID gène
9118
UniProt
Q16352
Vus récemment
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