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CA1 Kit ELISA

CA1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882025
  • Antigène Voir toutes CA1 Kits ELISA
    CA1 (Carbonic Anhydrase I (CA1))
    Reactivité
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CA1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CA1 Kits ELISA
    CA1 (Carbonic Anhydrase I (CA1))
    Autre désignation
    CA1 (CA1 Produits)
    Synonymes
    CA-I Kit ELISA, CAB Kit ELISA, Car1 Kit ELISA, AW555628 Kit ELISA, Ca1 Kit ELISA, Car-1 Kit ELISA, BG:DS00941.1 Kit ELISA, CAH Kit ELISA, CG7820 Kit ELISA, Dmel\\CG7820 Kit ELISA, Gh7 Kit ELISA, ARABIDOPSIS THALIANA SALICYLIC ACID-BINDING PROTEIN 3 Kit ELISA, ATBCA1 Kit ELISA, ATSABP3 Kit ELISA, BETA CARBONIC ANHYDRASE 1 Kit ELISA, F4P13.5 Kit ELISA, F4P13_5 Kit ELISA, SABP3 Kit ELISA, SALICYLIC ACID-BINDING PROTEIN 3 Kit ELISA, carbonic anhydrase 1 Kit ELISA, CA1 Kit ELISA, LOC100135826 Kit ELISA, GB15888 Kit ELISA, carbonic anhydrase 1 Kit ELISA, Carbonic anhydrase 1 Kit ELISA, carbonic anhydrase I Kit ELISA, carbonic anhydrase Kit ELISA, CA1 Kit ELISA, Car1 Kit ELISA, CAH1 Kit ELISA, LOC100135826 Kit ELISA, LOC408827 Kit ELISA, Tsp_05114 Kit ELISA, LOC100153915 Kit ELISA, LOC100050192 Kit ELISA, Ca1 Kit ELISA
    ID gène
    759
    UniProt
    P00915
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