CA5B Kit ELISA

N° du produit ABIN4882055

Détail du produit CA5B Kit ELISA

Antigène: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human CA5B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))

Autre désignation: CA5B (CA5B Produits)

Synonymes: CA-VB Kit ELISA, 7330410H16Rik Kit ELISA, CAVB Kit ELISA, Ca5b Kit ELISA, CarVb Kit ELISA, D730005F19Rik Kit ELISA, zgc:171653 Kit ELISA, CA5B Kit ELISA, ca5b Kit ELISA, carbonic anhydrase 5B Kit ELISA, carbonic anhydrase 5b, mitochondrial Kit ELISA, carbonic anhydrase Va Kit ELISA, carbonic anhydrase 5A Kit ELISA, carbonic anhydrase 5B, mitochondrial Kit ELISA, carbonic anhydrase VB, mitochondrial L homeolog Kit ELISA, CA5B Kit ELISA, Car5b Kit ELISA, Ca5b Kit ELISA, ca5a Kit ELISA, CA5A Kit ELISA, LOC100051095 Kit ELISA, ca5b.L Kit ELISA

ID gène: 11238

UniProt: Q9Y2D0