CANT1 Kit ELISA

N° du produit ABIN4882079

Détail du produit CANT1 Kit ELISA

Antigène: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human CANT1/SCAN-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human FABP4.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CANT1 (Calcium Activated Nucleotidase 1 (CANT1))

Autre désignation: CANT1 (CANT1 Produits)

Synonymes: DBQD Kit ELISA, SCAN-1 Kit ELISA, SCAN1 Kit ELISA, SHAPY Kit ELISA, 5830420C20Rik Kit ELISA, Apy1h Kit ELISA, D11Bwg0554e Kit ELISA, Entpd8 Kit ELISA, Shapy Kit ELISA, srapy Kit ELISA, apyrase Kit ELISA, xapy Kit ELISA, cant1 Kit ELISA, entpd8 Kit ELISA, zgc:85724 Kit ELISA, CANT1 Kit ELISA, zgc:101021 Kit ELISA, calcium activated nucleotidase 1 Kit ELISA, calcium activated nucleotidase 1 L homeolog Kit ELISA, calcium activated nucleotidase 1a Kit ELISA, calcium activated nucleotidase 1b Kit ELISA, CANT1 Kit ELISA, Cant1 Kit ELISA, cant1.L Kit ELISA, cant1 Kit ELISA, cant1a Kit ELISA, LOAG_01305 Kit ELISA, cant1b Kit ELISA

ID gène: 124583

UniProt: Q8WVQ1