CTSC Kit ELISA

N° du produit ABIN4882112

Détail du produit CTSC Kit ELISA

Antigène: CTSC (Cathepsin C (CTSC))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Cathepsin C ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Cathepsin C.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CTSC (Cathepsin C (CTSC))

Autre désignation: Cathepsin C (CTSC Produits)

Synonymes: CPPI Kit ELISA, DPP-I Kit ELISA, DPP1 Kit ELISA, DPPI Kit ELISA, HMS Kit ELISA, JP Kit ELISA, JPD Kit ELISA, PALS Kit ELISA, PDON1 Kit ELISA, PLS Kit ELISA, MGC69126 Kit ELISA, MGC107782 Kit ELISA, AI047818 Kit ELISA, CATC Kit ELISA, cb912 Kit ELISA, ik:tdsubc_1h2 Kit ELISA, sb:cb146 Kit ELISA, wu:fb34g12 Kit ELISA, wu:fj58d01 Kit ELISA, cathepsin C Kit ELISA, cathepsin C L homeolog Kit ELISA, cathepsin CPC1 Kit ELISA, CTSC Kit ELISA, ctsc.L Kit ELISA, ctsc Kit ELISA, TP03_0357 Kit ELISA, CMU_003360 Kit ELISA, CPC1 Kit ELISA, NAEGRDRAFT_80477 Kit ELISA, NAEGRDRAFT_37577 Kit ELISA, Ctsc Kit ELISA, Smp_183330 Kit ELISA

ID gène: 1075

UniProt: P53634