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CDON Kit ELISA

CDON Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882296
  • Antigène Voir toutes CDON Kits ELISA
    CDON (Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))
    Reactivité
    • 2
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CDO ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human CDO. Other species not yet determined.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CDON Kits ELISA
    CDON (Cell Adhesion Molecule-Related/down-Regulated By Oncogenes (CDON))
    Autre désignation
    CDO (CDON Produits)
    Synonymes
    CDO Kit ELISA, CDON1 Kit ELISA, HPE11 Kit ELISA, ORCAM Kit ELISA, Cdo Kit ELISA, Cd0 Kit ELISA, Orcam Kit ELISA, cb507 Kit ELISA, wu:fa10c03 Kit ELISA, wu:fb94b11 Kit ELISA, zgc:158240 Kit ELISA, cdon-A Kit ELISA, cell adhesion associated, oncogene regulated Kit ELISA, cell adhesion molecule-related/down-regulated by oncogenes Kit ELISA, cell adhesion associated, oncogene regulated L homeolog Kit ELISA, CDON Kit ELISA, Cdon Kit ELISA, cdon Kit ELISA, cdon.L Kit ELISA
    ID gène
    1036
    UniProt
    Q16878
    Pathways
    Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Embryonic Body Morphogenesis
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