CHMP2B Kit ELISA

N° du produit ABIN4882336

Détail du produit CHMP2B Kit ELISA

Antigène: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human CHMP2B ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Kallikrein 1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CHMP2B (Charged Multivesicular Body Protein 2B (CHMP2B))

Autre désignation: CHMP2B (CHMP2B Produits)

Synonymes: ALS17 Kit ELISA, CHMP2.5 Kit ELISA, DMT1 Kit ELISA, VPS2-2 Kit ELISA, VPS2B Kit ELISA, 1190006E07Rik Kit ELISA, RGD1306781 Kit ELISA, chmp2b Kit ELISA, chmp2bl Kit ELISA, zgc:77025 Kit ELISA, si:ch211-224m9.5 Kit ELISA, CHMP2B Kit ELISA, cg4618 Kit ELISA, chmp2.5 Kit ELISA, dmt1 Kit ELISA, vps2-2 Kit ELISA, vps2b Kit ELISA, CHMP2b-B Kit ELISA, chmp2b-a Kit ELISA, chmp2b-b Kit ELISA, charged multivesicular body protein 2B Kit ELISA, charged multivesicular body protein 2Bb Kit ELISA, charged multivesicular body protein 2Ba Kit ELISA, charged multivesicular body protein 2B pseudogene Kit ELISA, charged multivesicular body protein 2B S homeolog Kit ELISA, charged multivesicular body protein 2B L homeolog Kit ELISA, CHMP2B Kit ELISA, Chmp2b Kit ELISA, chmp2bb Kit ELISA, chmp2ba Kit ELISA, LOC747711 Kit ELISA, chmp2b.S Kit ELISA, chmp2b.L Kit ELISA

ID gène: 25978

UniProt: Q9UQN3