CILP Kit ELISA

N° du produit ABIN4882357

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain CILP. Il a été cité dans 1 publication.

Aperçu rapide pour CILP Kit ELISA (ABIN4882357)

Antigène: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.053 ng/mL - 13 ng/mL

Application: ELISA

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Détail du produit CILP Kit ELISA

Seuil minimal de détection: 0.053 ng/mL

Fonction: Human CILP-1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human 4-1BB Ligand, Activin RIIB, Aminopeptidase P2, BAMBI, BOC, Brevican, Carbonic Anhydrase XII, Carboxypeptidase A2, CD300c (LMIR2), CD320, CDNF, CDO, CHST1, CHST4, CNTF R alpha, CRIM1, CRTAC1, CXADR, Dopa Decarboxylase (DDC), DPPII (QPP, DPP7), DSPG3, EMR2, FCAR (CD89), FCRL1 (FcRH1), FCRL2 (FcRH2), Gas6, GPR56, GPVI, Hepsin, ILT2 (CD85j), Jagged 2, Kirrel3 (NEPH2), KLF4, LAIR1, LAMP, LAMP1 (CD107a), MDGA1, MIS RII, Neurexin 3.

Sensibilité: 0.05 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 2 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour CILP Kit ELISA (ABIN4882357)

Weidenhammer, Prausmüller, Partsch, Spinka, Luckerbauer, Larch, Arfsten, Abdel Mawgoud, Bartko, Goliasch, Kastl, Hengstenberg, Hülsmann, Pavo: "CILP-1 Is a Biomarker for Backward Failure and Right Ventricular Dysfunction in HFrEF." dans: Cells, Vol. 12, Issue 24, (2024) (PubMed).

Détail du antigène

Antigène: CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))

Autre désignation: CILP-1

Sujet:

Gene Symbols: CILP, UNQ602, PRO1188

Protein Name (Synonyms): Cartilage intermediate layer protein 1 (CILP-1) (Cartilage intermediate-layer protein) [Cleaved into: Cartilage intermediate layer protein 1 C1 Cartilage intermediate layer protein 1 C2

ID gène: 8483

UniProt: O75339