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CRHBP Kit ELISA

CRHBP Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882475
  • Antigène Voir toutes CRHBP Kits ELISA
    CRHBP (Corticotropin Releasing Hormone Binding Protein (CRHBP))
    Reactivité
    • 3
    • 2
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CRHBP ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CRHBP Kits ELISA
    CRHBP (Corticotropin Releasing Hormone Binding Protein (CRHBP))
    Autre désignation
    CRHBP (CRHBP Produits)
    Synonymes
    crh-BP Kit ELISA, GB14094 Kit ELISA, zgc:91905 Kit ELISA, CRHBP Kit ELISA, CRH-BP Kit ELISA, CRF-BP Kit ELISA, CRFBP Kit ELISA, Crfbp Kit ELISA, crhbp-A Kit ELISA, gene E Kit ELISA, corticotropin releasing hormone binding protein Kit ELISA, corticotropin releasing hormone binding protein S homeolog Kit ELISA, CRHBP Kit ELISA, crhbp Kit ELISA, crh-BP Kit ELISA, Crhbp Kit ELISA, crhbp.S Kit ELISA
    ID gène
    1393
    UniProt
    P24387
    Pathways
    Negative Regulation of Hormone Secretion
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