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CRISP2 Kit ELISA

CRISP2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882481
  • Antigène Voir toutes CRISP2 Kits ELISA
    CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human CRISP-2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CRISP2 Kits ELISA
    CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))
    Autre désignation
    CRISP-2 (CRISP2 Produits)
    Synonymes
    ct36 Kit ELISA, tpx1 Kit ELISA, tsp1 Kit ELISA, crisp Kit ELISA, gapdl5 Kit ELISA, crisp-2 Kit ELISA, CRISP3 Kit ELISA, CRISP2 Kit ELISA, CRISP-2 Kit ELISA, GAPDL5 Kit ELISA, Tpx-1 Kit ELISA, Tpx1 Kit ELISA, Crisp2l Kit ELISA, CT36 Kit ELISA, TPX1 Kit ELISA, TSP1 Kit ELISA, CRISP-3 Kit ELISA, CRISP-1 Kit ELISA, Crisp2 Kit ELISA, cysteine-rich secretory protein 2 L homeolog Kit ELISA, cysteine rich secretory protein 2 Kit ELISA, cysteine-rich secretory protein 2 Kit ELISA, crisp2.L Kit ELISA, CRISP2 Kit ELISA, Crisp2 Kit ELISA
    ID gène
    7180
    UniProt
    P16562
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