CSTB Kit ELISA

N° du produit ABIN4882533

Kit ELISA CSTB Humain, Colorimetric test pour la quantification de Humain CSTB.

Aperçu rapide pour CSTB Kit ELISA (ABIN4882533)

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 4 pg/mL - 1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit CSTB Kit ELISA

Seuil minimal de détection: 4 pg/mL

Fonction: Human Cystatin B/CSTB ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Analytical Method: Quantitative

Specificité: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAM-12, ADAM-8, B7-H3 (CD276), BMPR-IB, Cadherin-13, Cadherin-4, CD155 (PVR), CD229 (SLAMF3), CD48 (SLAMF2), CD58 (LFA-3), CD84 (SLAMF5), CD99, CEACAM-5, Coagulation Factor XIV (Protein C), Cystatin A, Cystatin E/M, Desmoglein-2, DR3 (TNFRSF25), ErbB4 (HER4), ESAM, FGF-21, Galectin-2, Galectin-9, ICOS, JAM-A (CD321/F11R), JAM-B (VE-JAM/CD322), Kallikrein 5, Midkine, Pentraxin-3 (TSG-14), Pref-1 (DLK1/FA1), Siglec-10, SLAM (CD150), SP-D, Syndecan-4, Testican 2 (SPOCK2), TIM-3 (KIM-3), TLR4, TRAIL (TNFSF10), ULBP-1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Suggested Plasma Dilution: 15 fold

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: CSTB (Cystatin B (Stefin B) (CSTB))

Autre désignation: Cystatin B

Sujet:

Gene Symbols: CST6, CSTB, STFB

Protein Name (Synonyms): Cystatin-B (CPI-B) (Liver thiol proteinase inhibitor) (Stefin-B)

ID gène: 1476

UniProt: P04080

Pathways: Response to Water Deprivation