DSCAM Kit ELISA

N° du produit ABIN4882660

Détail du produit DSCAM Kit ELISA

Antigène: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human DSCAM ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))

Autre désignation: DSCAM (DSCAM Produits)

Synonymes: CHD2-42 Kit ELISA, CHD2-52 Kit ELISA, Dscam Kit ELISA, DSCAM Kit ELISA, chd2-42 Kit ELISA, chd2-52 Kit ELISA, dscam Kit ELISA, GB15141 Kit ELISA, 4932410A21Rik Kit ELISA, 43Bc Kit ELISA, CG17800 Kit ELISA, CT39257 Kit ELISA, DScam Kit ELISA, DmDscam Kit ELISA, Dm_2R:13612 Kit ELISA, Dmel\\CG17800 Kit ELISA, Dscam-hv Kit ELISA, Dscam1 Kit ELISA, FBgn0033159 Kit ELISA, Neu1 Kit ELISA, dScam Kit ELISA, l(2)05518 Kit ELISA, l(2)43Bc Kit ELISA, p270 Kit ELISA, DS cell adhesion molecule Kit ELISA, Down syndrome cell adhesion molecule Kit ELISA, Down syndrome cell adhesion molecule a Kit ELISA, Down syndrome cell adhesion molecule 1 Kit ELISA, Down syndrome cell adhesion molecule-like protein Dscam2 Kit ELISA, down syndrome cell adhesion molecule Kit ELISA, Down syndrome cell adhesion molecule L homeolog Kit ELISA, DSCAM Kit ELISA, Dscam Kit ELISA, dscam Kit ELISA, dscama Kit ELISA, Dscam1 Kit ELISA, LOC5573626 Kit ELISA, CpipJ_CPIJ006173 Kit ELISA, dscam.L Kit ELISA

ID gène: 1826

UniProt: O60469

Pathways: Regulation of Cell Size