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DYRK3 Kit ELISA

DYRK3 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882678
  • Antigène Tous les produits DYRK3
    DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human DYRK3 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human SMAD3.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Tous les produits DYRK3
    DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))
    Autre désignation
    DYRK3 (DYRK3 Produits)
    Synonymes
    DYRK5 Kit ELISA, RED Kit ELISA, REDK Kit ELISA, hYAK3-2 Kit ELISA, BC006704 Kit ELISA, DYRK3 Kit ELISA, MGC89944 Kit ELISA, dual specificity tyrosine phosphorylation regulated kinase 3 Kit ELISA, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 Kit ELISA, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 3 Kit ELISA, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 3 L homeolog Kit ELISA, DYRK3 Kit ELISA, Dyrk3 Kit ELISA, dyrk3 Kit ELISA, dyrk3.L Kit ELISA
    ID gène
    8444
    UniProt
    O43781
    Pathways
    Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha
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