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TMPRSS15 Kit ELISA

TMPRSS15 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4882731
  • Antigène Voir toutes TMPRSS15 Kits ELISA
    TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
    Reactivité
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human Enteropeptidase ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes TMPRSS15 Kits ELISA
    TMPRSS15 (Transmembrane Protease, serine 15 (TMPRSS15))
    Autre désignation
    Enteropeptidase (TMPRSS15 Produits)
    Synonymes
    CG3066 Kit ELISA, Dmel\\CG3066 Kit ELISA, MP2 Kit ELISA, PAE1 Kit ELISA, SP7 Kit ELISA, anon-Ryu Kit ELISA, proPO-AE Kit ELISA, sp7 Kit ELISA, NV14771 Kit ELISA, PRSS7 Kit ELISA, ENTK Kit ELISA, Entk Kit ELISA, Prss7 Kit ELISA, Serine protease 7 Kit ELISA, serine protease 7 Kit ELISA, transmembrane protease, serine 7 Kit ELISA, transmembrane protease, serine 15 Kit ELISA, Sp7 Kit ELISA, LOC733125 Kit ELISA, SP7 Kit ELISA, TMPRSS7 Kit ELISA, TMPRSS15 Kit ELISA, Tmprss15 Kit ELISA
    ID gène
    5651
    UniProt
    P98073
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