FOXJ1 Kit ELISA

N° du produit ABIN4882926

Détail du produit FOXJ1 Kit ELISA

Antigène: FOXJ1 (Forkhead Box J1 (FOXJ1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Custom Human FoxJ1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes .

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: FOXJ1 (Forkhead Box J1 (FOXJ1))

Autre désignation: FoxJ1 (FOXJ1 Produits)

Synonymes: FOXJ1 Kit ELISA, foxJ1 Kit ELISA, foxJ Kit ELISA, FKHL-13 Kit ELISA, HFH-4 Kit ELISA, Hfh4 Kit ELISA, Hfh-4 Kit ELISA, FKHL13 Kit ELISA, HFH4 Kit ELISA, fj67h07 Kit ELISA, wu:fj67h07 Kit ELISA, zgc:101713 Kit ELISA, foxj1 Kit ELISA, id:ibd5134 Kit ELISA, zgc:153050 Kit ELISA, FoxJ1-A Kit ELISA, FoxJ1a Kit ELISA, foxj1-a Kit ELISA, foxj1-b Kit ELISA, foxj1a Kit ELISA, hfh-4 Kit ELISA, hfh4 Kit ELISA, xFKH5 Kit ELISA, xFoxJ1 Kit ELISA, xfoxj1' Kit ELISA, forkhead transcription factor J1 Kit ELISA, forkhead box J1 Kit ELISA, forkhead box J1b Kit ELISA, forkhead box J1a Kit ELISA, forkhead box J1 S homeolog Kit ELISA, FoxJ1 Kit ELISA, FOXJ1 Kit ELISA, foxj1 Kit ELISA, foxJ1 Kit ELISA, Foxj1 Kit ELISA, foxj1b Kit ELISA, foxj1a Kit ELISA, foxj1.S Kit ELISA

ID gène: 2302

UniProt: Q92949

Pathways: Regulation of Leukocyte Mediated Immunity