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GATA2 Kit ELISA

GATA2 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN4883020
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    GATA2 (GATA Binding Protein 2 (GATA2))
    Reactivité
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    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Human GATA-2 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes .
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes GATA2 Kits ELISA
    GATA2 (GATA Binding Protein 2 (GATA2))
    Autre désignation
    GATA-2 (GATA2 Produits)
    Synonymes
    DCML Kit ELISA, MONOMAC Kit ELISA, NFE1B Kit ELISA, Gata-2 Kit ELISA, xgata-2 Kit ELISA, xgata2 Kit ELISA, GATA-2 Kit ELISA, NF-E1b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2 Kit ELISA, zg2 Kit ELISA, zgc:91840 Kit ELISA, GATA binding protein 2 Kit ELISA, GATA binding protein 2 L homeolog Kit ELISA, amidase Kit ELISA, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A Kit ELISA, hypothetical protein Kit ELISA, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) Kit ELISA, GATA2 transcription factor Kit ELISA, putative gata binding factor Kit ELISA, GATA binding protein 2a Kit ELISA, GATA binding protein 2b Kit ELISA, GATA2 Kit ELISA, Gata2 Kit ELISA, gata2.L Kit ELISA, RR_RS20245 Kit ELISA, gatA2 Kit ELISA, Smp_018590 Kit ELISA, gata2a Kit ELISA, gata2b Kit ELISA
    ID gène
    2624
    UniProt
    P23769
    Pathways
    Stem Cell Maintenance
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